Kurşun asetat maruziyeti sonucu oluşan nefrotoksisiteye karşı mirisetin, kaemferol, fisetin ve galanginin koruyucu rolünün araştırılması

AYHAN YİĞİT

Kurşun asetat maruziyeti sonucu oluşan nefrotoksisiteye karşı mirisetin, kaemferol, fisetin ve galanginin koruyucu rolünün araştırılması

Thesis Type: Postgraduate

Institution Of The Thesis: Van Yüzüncü Yil University, Sağlık Bilimleri Enstitüsü, Temel Eczacılık Bilimleri (Yl) (Tezli), Turkey

Approval Date: 2021

Thesis Language: Turkish

Student: AYHAN YİĞİT

Supervisor: Mehmet Berköz

Open Archive Collection: AVESIS Open Access Collection

Abstract:

In this study, it was tried to reveal to what extent the administration of myricetin, kaemferol, fisetin and galangin, which are known to have antioxidant and anti-inflammatory effects, can prevent oxidative stress and inflammatory response in the kidney tissue in rats with experimental lead acetate (LA) induction. 70 rats were used in the study. Rats were randomly selected and divided into 10 groups. These groups are respectively; control, LA (100 mg/kg), LA+MYC100 (100 mg/kg), LA+MYC200 (200 mg/kg), LA+KMP100 (100 mg/kg), LA+KMP200 (200 mg/kg), LA+FST100 (100 mg/kg), LA+FST200 (200 mg/kg), LA+GLG100 (100 mg/kg) and LA+GLG200 (200 mg/kg) groups. All substances used in the study were given by gavage every day for 28 days. At the end of the study, serum was obtained from the blood of all animals taken by cardiac puncture under anesthesia, and kidney tissues were removed. The amounts of urea, creatinine and blood urea nitrogen in serum samples were measured in an autoanalyzer using a commercial kit. In kidney tissues, malondialdehyde (MDA) level to determine lipid peroxidation, protein carbonyl, total and free thiol levels to determine protein oxidation, 8-hydroxy deoxy guanosine (8-OH dG) level to determine DNA oxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GSH-Rx), paraoxonase (PON), aryl esterase (ARE) activities and reduced glutathione (GSH) level, and nitric oxide for observing the inflammatory response. (NOx) and prostaglandin E2 (PGE2) levels were measured. Statistical analysis of the study was performed using SPSS v16.0 statistical program. Obtained data were expressed as mean±standard deviation, and data with a p value of 0.05 or less were considered statistically significant. One-way analysis of variance (ANOVA) and Tukey test were used to evaluate biochemical parameters. It was observed that lead administration impaired kidney function tests, increased lipid peroxidation, protein and DNA oxidation and inflammatory response in kidney tissue, and weakened the antioxidant defense system (p<0.05). It was observed that myricetin and fisetin administration prevented oxidative stress and inflammation in kidney tissue (p<0.05). As a result, it was observed that especially high-dose myristein and fisetin applications could prevent nephrotoxicity, while kaemferol and galangin did not have a protective effect as desired. However, the use of lead should be limited as much as possible, and if it is to be used, necessary precautions should be taken to the maximum extent and its excessive release to the environment should be minimized.