Adıyaman İli Tütün Üretim Alanlarındaki Bazı Tütün Virüslerinin Multipleks RT-PCR Yöntemi İle Araştırılması ve Bazı Virüs izolatlarının Moleküler Karekterizasyonu


Thesis Type: Postgraduate

Institution Of The Thesis: Van Yüzüncü Yil University, Fen Bilimleri Enstitüsü, Bitki Koruma (Yl) (Tezli), Turkey

Approval Date: 2019

Thesis Language: Turkish

Student: Abidin GÜNAY

Principal Supervisor (For Co-Supervisor Theses): Mustafa Usta

Abstract:

Survey studies were conducted in 2018 in order to detect tobacco viruses (TMV, TEV, PVY, CMV and TVBMV) in tobacco production areas of Adıyaman province. Multiplex RT-PCR method was used to test the presence of tobacco viruses. Four hundred fifty fresh leaf samples were collected from tobacco fields in Adiyaman province. Of the 450 tobacco samples tested, 40 had CMV (8.8 %), 25 had TMV (5.5 %), and 1 had TMV + CMV infection (0.2 %). The overall infection rate of tobacco viruses in Adiyaman province was found to be 14.4%.According to the molecular tests, TEV, PVY, TVMBV viruses were not detected in the collected tobacco samples. Among the viruses detected in Adıyaman province, all of the coat protein genes of two randomly selected isolates (TMV 20, TMV 307, CMV 61and CMV 73) were cloned and molecularly characterized. TMV Adıyaman isolates showed similarity between 72.3 % and 99.17 % while CMV Adıyaman isolates showed similarity between 74.09-99.54 % at nucleotide level with other 19 different isolates in the World. Othervise, CMV Adıyaman isolates showed similarity between 74.09 % and 99.54 % at nucleotide level with other 20 different isolates in the world. In this study, CMV and TMV were first reported in tobacco production areas of Adiyaman province.