Akademik Veri Yönetim Sistemi
Furan M. A. (Yürütücü), ZARİFİ R.
Yükseköğretim Kurumları Destekli Proje, BAP Y.Lisans, 2024 - 2025
Mycorrhizae are symbiotic organisms that form mutualistic associations with
plant roots, facilitating plant-soil continuity. They play a vital role in improving soil
structure, enhancing plant tolerance to biotic and abiotic stresses, maintaining ecological
balance, and promoting sustainable agriculture. Mycorrhizal fungi colonize
approximately 85% of terrestrial plants and are key contributors to biodiversity and
ecosystem stability. These fungi differ in morphology, spore formation, and mycelial
architecture, and are taxonomically classified based on these features. Among them,
Arbuscular Mycorrhizal Fungi (AMF) represent a widely distributed and agriculturally
important group in the rhizosphere. As obligate biotrophs, AMF depend on host-derived
carbon while enhancing The process of water absorption, phosphate, nitrogen, Plus other
important nutrients via extraradical and intraradical hyphae, arbuscules, and root
apoplasts. Unlike other mycorrhizal types, AMF form tree-like arbuscules within root
cortical cells, acting as key sites for nutrient exchange between fungus and host.
In this study, universal ITS1 and ITS4 primers were used to amplify the ITS
regions from genomic DNA isolated from AMF species maintained in culture stocks at
the Mycology Laboratory, Faculty of Agriculture, Van Yüzüncü Yıl University. The
amplified ITS1 and ITS4 regions of rDNA were analyzed using various bioinformatics
tools, including SnapGene, MEGA X, ITOL, Python ve Alifold Sever Viewer.
Phylogenetic analysis based on sequence alignments of the ITS1 and ITS4 regions
revealed that the secondary structure derived from these sequences is highly stable. Our
results demonstrate that when combined with phylogenetic tree analysis, ITS1 and ITS4
markers serve as effective molecular tools for the identification of five AMF species.