INVESTIGATION OF BIOFILM FORMING ABILITY IN ESCHERICHIA COLI ISOLATES ISOLATED FROM CHILDREN’S PARK


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Çalışkan D., Şavluk M., Kaşkatepe B.

3rd International Gazi Pharma Symposium Series (GPSS-2021), Ankara, Turkey, 8 - 10 September 2021, pp.93

  • Publication Type: Conference Paper / Summary Text
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.93
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Biofilm-producing bacteria has become a major public health problem worldwide. The biofilm structure makes the bacteria resistant to the host defense mechanism. Biofilm plaques detected in many medical devices used in the health sector cause infections arising from these devices. The biofilm formed by Escherichia coli consists of a bacterial colony composed of extracellular exopolysaccharide. Colonies from pure bacterial cultures will be transferred to 100µl TSB and incubated for 18 hours. The 0.5 McFarland standard will be diluted to obtain turbidity. 100µl will be added to the microplate wells by diluting with TSB medium. 100µl of 1:100 dilution of the prepared bacterial suspension will be added to these wells and incubated for 24 hours at 35°C. The wells will be washed 3 times with 200µl sterile distilled water and dried at 60°C. 200µl of 0.1% crystal violet will be added to dried microplates and stained for 15 minutes at room temperature. After staining, the wells will be washed with sterile distilled water and 200µl of 33% glacial acetic acid will be added after washing and the results will be measured in terms of optical density at 620nm in the spectrophotometer. Sterile TSB medium will be used for sterility control and wells that do contain Staphylococcus epidermidis and its formulations will be used for growth control. For E. coli strains, biofilm formation was interpreted according to the determined control groups. In conclusion; Low biofilm formation was observed in 10%, moderate in 70% and high in 20% of the strains.