Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, cilt.24, sa.2, ss.306-316, 2020 (Hakemli Dergi)
Microalgae can generally maintain the high amounts of biomass in the
wastewater and they can be converted from biomass to bio-butanol. Bio-butanol is
a liquid biofuel and it has significant physical and chemical properties. In this
study, we carried out bio-butanol production from Borodinellopsis texensis CCALA
892 grown in various concentrations of the municipal wastewater. Also, we
determined biochemical composition ratios of microalgae samples and studied the
some antioxidant enzymes such as catalase, superoxide dismutase and ascorbate
peroxidase. In the current study, bio-butanol was produced by the acetonebutanol-ethanol (ABE) fermentation method. The microalgae sample grown in
25% of wastewater had the highest biomass productivity among five wastewater
samples with 0.114 ± 0.002 g L-1d-1. The carbohydrate and protein concentrations
of control group increased day by day and their values reached stationary phases
at seven days. The sample grown in 25% of wastewater had the highest
carbohydrate concentration with 0.30 g L-1 and protein concentration with 0.35 g
L-1 at the stationary phase. The maximum enzyme activities for catalase,
superoxide dismutase and ascorbate peroxidase were 15.33 ± 0.88, 8.67 ± 0.67 and
33 ± 1.53 µmole/mg, respectively at 25% of wastewater. In addition, bio-butanol
content of B. texensis CCALA 892 was 3.63 ± 0.21 g L-1 and its bio-butanol yield was
found as 0.18 ± 0.011 g/g sugar. In the next study, we can examine large scale
butanol production.