Biomass and Bio-butanol Production from Borodinellopsis texensis CCALA 892 in Synthetic Wastewater: Determination of Biochemical Composition


Onay M.

Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, cilt.24, sa.2, ss.306-316, 2020 (Hakemli Dergi)

Özet

Microalgae can generally maintain the high amounts of biomass in the wastewater and they can be converted from biomass to bio-butanol. Bio-butanol is a liquid biofuel and it has significant physical and chemical properties. In this study, we carried out bio-butanol production from Borodinellopsis texensis CCALA 892 grown in various concentrations of the municipal wastewater. Also, we determined biochemical composition ratios of microalgae samples and studied the some antioxidant enzymes such as catalase, superoxide dismutase and ascorbate peroxidase. In the current study, bio-butanol was produced by the acetonebutanol-ethanol (ABE) fermentation method. The microalgae sample grown in 25% of wastewater had the highest biomass productivity among five wastewater samples with 0.114 ± 0.002 g L-1d-1. The carbohydrate and protein concentrations of control group increased day by day and their values reached stationary phases at seven days. The sample grown in 25% of wastewater had the highest carbohydrate concentration with 0.30 g L-1 and protein concentration with 0.35 g L-1 at the stationary phase. The maximum enzyme activities for catalase, superoxide dismutase and ascorbate peroxidase were 15.33 ± 0.88, 8.67 ± 0.67 and 33 ± 1.53 µmole/mg, respectively at 25% of wastewater. In addition, bio-butanol content of B. texensis CCALA 892 was 3.63 ± 0.21 g L-1 and its bio-butanol yield was found as 0.18 ± 0.011 g/g sugar. In the next study, we can examine large scale butanol production.