Characterization and Identification of Important Phytoplasma Diseases in Solanacea Family in Van Province


Usta M., Sipahioğlu H. M., Güller A.

II.Internatonal Igdır Sypmposym, Iğdır, Türkiye, 9 - 11 Ekim 2017, ss.77

  • Yayın Türü: Bildiri / Özet Bildiri
  • Basıldığı Şehir: Iğdır
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.77
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

There are important cultivated plants such as tomatoes, potatoes, peppers and eggplants among Solacacea family plants. Phytoplasma-like symptoms were observed in Solanacea
family plants in Van province. The most important signs observed include violent dwarfism, large buds, small leaf formation, curling of leaves, bruising, witch broom appearance,
rosetting, and small leafiness. DNA fragments of approximately 1250 bp were amplified as a result of the Nested-PCR test performed with R16mF2 / R16mR1 and R16F2n / R16R2 primer pairs in 200 different plant samples. 16S rDNA fragments of phytoplasma agents were amplified in the PCR reaction using genomic DNA isolated from leaves of plants with and without symptoms. DNA fragments of 1.25 kb were obtained from 200 in 19 samples tested by PCR. A randomly selected 2 isolates from each of the positive samples was cloned into a  pGEM T- Easy plasmid vector. BLAST and RFLP analyzes of the 16S rDNA sequence were performed after the purified recombinant plasmid DNA was sequenced bidirectionally. It has  been found out that tomato isolates showing severe phytoplasma symptoms has '
Candidatus Phytoplasma solani' (GenBank Accession number: KY579358) and 'Candidatus Phytoplasma trifolii'(GenBank Accession number: KY564268), pepper isolates has ‘Candidatus Phytoplasma solani’(GenBank Accession number: MF564267) and ‘Candidatus Phytoplasma trifolii’ (GenBank Accession number: MF564266) and eggplant has 'Candidatus Phytoplasma solani (GenBank Accession number: KR080212, KT595210). According to the authors' information, this report is the first report of phytoplasmas of Ca. P. trifolii naturally infecting tomato and eggplant in Turkey.