Stereological estimation of the upper incisor dental pulp volume in four week-old rats exposed to diclofenac sodium prenatal period

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Rağbetli M. Ç. , Aydoğan M. Ç. , Koyun N.

2nd ASIAN AND AFRICAN STEREOLOGY CONGRESS, Dubai, United Arab Emirates, 3 - 05 December 2015, pp.27

  • Publication Type: Conference Paper / Summary Text
  • City: Dubai
  • Country: United Arab Emirates
  • Page Numbers: pp.27
  • Van Yüzüncü Yıl University Affiliated: Yes


The  aim  of  our  study  is  investigation  of  puppy  tooth’s  enamel,  dentin, 

cementum and pulp tissues volume that are exposed t o diclofenac sodium 

that is analgesic, anti-inflammatory and antipyretic on postpartum 4 weeks 

rats  by  stereological  methods.  Pregnant  rats  were  kept  in  a  room  with 

normal  light  and  dark  cycles  (21±2ºC)  and  fed  with  normal  diet.  The 

injections in the sham (1cc SF) and the experimental group (1mg/kg) were 

started  on  the  fifty  day  of  pregnancy  and  applied  for  15  days.  On  the 

postnatal 28th day (between 3-4 weeks) the male and female offspring were 

separated  into  different  cages. In  our  study,  totally,  36  Wistar  albino  rats 

were  used  in  the  experimental,  sham  and  control  groups  each  having  six 

male  and  six  female  rats.  The  animals  are  4  weeks  young  they  were 

perfused  under  deep  anesthesia  and  teeth  were  removed.  The  teeth  were 

decalcified.  Teeth  were  embedded  in  paraffin  after  routine  histological 

procedures. Tissue sections of about 5 microns thick were taken at regular 

intervals. The first section was chosen at random and approximately 10th 

section pairs were taken. The sections were stained with hematoxylin and 

eosin  staining and  modified  method  of  Cavalieri’s  principle  was  used  for 

stereological  analysis.  Pulp,  dentin,  odontoblasts  all  dental  layer  tissue 

volumes were measured with pointed field scale. As a result it was found 

that  prenatally  applied  diclofenak  sodium  did  not  cause  a  statistically 

significant change in the day 28 young rat enamel, dentin, cementum, pulp 

and periodontium layer of teeth and total tissues volume compared to the 

control animals. In this study do not support the suggestion that diclofenac 

sodium  is  interferes  with  development  of  the  tooth  pulp. Further  doses 

studies in these tissues are needed due to using in pregnancy.