Akademik Veri Yönetim Sistemi
2nd ASIAN AND AFRICAN STEREOLOGY CONGRESS, Dubai, Birleşik Arap Emirlikleri, 3 - 05 Aralık 2015, ss.27
The aim of our study is investigation of puppy tooth’s enamel, dentin,
cementum and pulp tissues volume that are exposed t o diclofenac sodium
that is analgesic, anti-inflammatory and antipyretic on postpartum 4 weeks
rats by stereological methods. Pregnant rats were kept in a room with
normal light and dark cycles (21±2ºC) and fed with normal diet. The
injections in the sham (1cc SF) and the experimental group (1mg/kg) were
started on the fifty day of pregnancy and applied for 15 days. On the
postnatal 28th day (between 3-4 weeks) the male and female offspring were
separated into different cages. In our study, totally, 36 Wistar albino rats
were used in the experimental, sham and control groups each having six
male and six female rats. The animals are 4 weeks young they were
perfused under deep anesthesia and teeth were removed. The teeth were
decalcified. Teeth were embedded in paraffin after routine histological
procedures. Tissue sections of about 5 microns thick were taken at regular
intervals. The first section was chosen at random and approximately 10th
section pairs were taken. The sections were stained with hematoxylin and
eosin staining and modified method of Cavalieri’s principle was used for
stereological analysis. Pulp, dentin, odontoblasts all dental layer tissue
volumes were measured with pointed field scale. As a result it was found
that prenatally applied diclofenak sodium did not cause a statistically
significant change in the day 28 young rat enamel, dentin, cementum, pulp
and periodontium layer of teeth and total tissues volume compared to the
control animals. In this study do not support the suggestion that diclofenac
sodium is interferes with development of the tooth pulp. Further doses
studies in these tissues are needed due to using in pregnancy.