The impact of lycopene on necrotik pathway in fluoride applied kidney epithelial cell line


Çetin S., Yüksek V., Usta A., Dede S., Taşpınar M.

Ist International Congress of The Turkic World on Health and Natural Sciences (1.ITRCHSNSC-2019), Osh, Kyrgyzstan, 21 - 24 April 2019, vol.1, no.1, pp.155-156

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Osh
  • Country: Kyrgyzstan
  • Page Numbers: pp.155-156

Abstract

Abstract The aim of the present study was to investigate the cellular damage in renal cell line included in the fluoride metabolism and affected by toxicity and to determine the role of lycopene on necrotic genes in prevention of these mechanisms. The renal epithelial cells were proliferated in RPMI 1640 medium that contained 10% FBS, 1% LGlutamine (2mM) and 1% penicillin/streptomycin. The cytotoxicity of NaF and lycopene (IC50) was determined by MTT. Total RNA and cDNA isolation were conducted in NaF and lycopene incubated cells at the dose and duration of cytotoxic values and the expression of target genes was investigated with RT-PCR. In MTT viability test, lycopene dose that increased proliferation (1µM) and the IC50 value for Naf on the 24th hour was determined as 3200 µM. It was determined that the Ripk1 gene expression did not change in the lycopene administered group after 24 hours, however it increased by 2.8 times in the fluoride and fluoride + lycopene administered groups. A significant difference was found between the gene expression pattern of the groups. It was determined that the Ripk3 gene expression did not change in the lycopene group on the 24th hour, however it was found to be 9 times higher in the fluoride and fluoride + lycopene administered groups. The change on the 24th hour was significantly higher when compared to the other groups. In conclusion, it can be suggested that necrotic genes became more active in NaF-induced cell death in the present study. Keywords: MTT, NaF, lycopene, cell culture