This study aims to investigate the antidiabetic, antimicrobial, DNA damage, and lipid peroxidation prevention activity of ZnO NPs/Rr formed as a result of the interaction of Rheum ribes (R.ribes) plant with ZnO. The ZnO NPs/Rr obtained as a result of the reaction were confirmed using high-reliability characterization methods. According to the data obtained as a result of the study, it is seen that the activity of ZnO NPs/Rr to prevent lipid peroxidation is quite strong. Lipid peroxidation inhibition activity of ZnO NPs/Rr at the highest concentration of 250 mu g/ml was calculated as % 89.1028. It was observed that ZnO NPs/Rr prevented DNA damage by % 92.1240 at the highest concentration of 100 mu g/ml. It was determined that the antidiabetic effect of ZnO NPs/Rr formed by ZnO of R. ribes plant, which is used as a medicinal plant as an antidiabetic, was significant. It appears to have a strong antidiabetic property compared to the positive control acarbose. In our current study, it was observed that ZnO NPs/Rr formed zones ranging from 8 +/- 3.0 to 21 +/- 4.5 against Gram-positive and Gram-negative microorganisms. It has been determined that ZnO nanoparticles have an antibacterial effect.