Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Van Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Zootekni (Yl) (Tezli), Türkiye
Tezin Onay Tarihi: 2018
Tezin Dili: İngilizce
Öğrenci: Rasul MAHMOOD
Danışman: Hasan Koyun
Özet:
Genetic
markers and their applications to animal molacular genetics have commonly been
used to determine polymorphisms. Restriction Fragment Length Polymorphism
(RFLP) is a molecular method of genetic
analysis exploiting variations in homologous DNA
sequences, consequently being considered as one of the informative genetic
markers.The growth hormone (GH) gene
plays major roles in growth regulation and development of higher organisms and
the growth traits are complex traits involving multiple genes, loci and
interactions. The aim of the research was to detect GH polymorphisms in Norduz
sheep. Genomic DNAs were isolated from blood samples of Norduz sheep and GH1
primers based on the 5 'flanking sequence of the sheep GH1 gene (Gene Bank
Accession Number: D00476) designed by (Hua et al., 2009) and GH gene designed
by (Kuulasma, 2002) were amplified. The amplified PCR product with the length
of 422 bp for GH1 locus and 934 bp for GH locus. Both loci were digested with HaeIII restriction enzyme and the
results displayed the presence of GH1/HaeIII
homozygous AA genotype with fragments 366 bp and 56 bp and heterozyogous AB
genotype with fragments 422 bp and 366 bp.
However, homozygous BB genotype did not appear. Considering GH/HaeIII digestion, all expected
genotypes AA, AB, BB were detected. Additionally, the population under
investigation did not exhibite to be in Hardy-Weinberg
equilibrium (HWE) for both loci. The present study concluded that GH1and GH/HaeIII could be used as a
genetic marker in sheep for growth characters.
Keywords: Growth hormone,
(GH1 and GH) genes, HaeIII digestion,
Norduz sheep, PCR-RFLP.