Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Van Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Zootekni (Yl) (Tezli) (Siirt Ün.ortak), Türkiye
Tezin Onay Tarihi: 2020
Tezin Dili: İngilizce
Öğrenci: NIZAR IBRAHIM YOUSIF
Danışman: Hasan Koyun
Özet:
Genetic markers or DNA markers
are valuable in distinguishing genetic variations between individuals. The
advancement of DNA-based DNA markers has had a progressive effect on hereditary
studies. Recently, the application of DNA-markers has commonly been used to
determine polymorphism in human, animal, aquaculture, and plant fields. There
are different types of markers that can be used to identify polymorphism
in animals, but the most commonly used ones are RAPD, AFLP, RFLP, mini
and micro-satellites and SNPs. The aim of the research was to identify the
genetic polymorphisms in growth hormone gene (GH) by using its three different
loci named (GHY1, GHY2, and GHY3) in the genome of Norduz sheep. In this
research, the PCR-RFLP technique was performed to determine genetic
variations in the growth hormone (GH) genes in Norduz sheep. First,
genomic DNA were purified from 50 individuals of Norduz sheep, then three sets
of primers were chosen to amplify loci of GHY1, GHY2, and GHY. The lengths of
PCR products were 599 bp, 690 bp, and 679 bp respectively. Furthermore, three
loci were digested with MspI, a DNA endonuclease enzyme. Results
displayed that there were no polymorphisms for three loci of GH. Only,
one particular genotype AB heterozygous was observed in all individuals whereas
genotypes AA and BB were not observed under this investigation. The results of
this study have suggested that further affords of the research in GH to detect
polymorphisms one should use SNPs which is more reliable technique to identify
polymorphisms.
Keywords: Growth hormone gene (GH),
Genetic Markers, Norduz Sheep, PCR-RFLP.