Van İlindeki Sokak Köpeklerinde Babesia Türlerinin Polimeraz Zincir Reaksiyonu Yöntemi ile Araştırılması


Tezin Türü: Yüksek Lisans

Tezin Yürütüldüğü Kurum: Van Yüzüncü Yıl Üniversitesi, Veteriner Fakültesi, Klinik Öncesi Bilimler Bölümü, Türkiye

Tezin Onay Tarihi: 2022

Tezin Dili: Türkçe

Öğrenci: Ömer SELÇİN

Danışman: Bekir Oğuz

Özet:

Canine babesiosis is a tick-borne protozoal disease caused by Babesia spp., which can cause hemolytic anemia, splenomegaly, thrombocytopenia and fever. As a result of molecular studies conducted in Turkey, the presence of B. canis, B. gibsoni, B. vogeli, B. rossi and B. vulpes (formerly B. microti-like/ Theileria annae) species has been revealed. To the best of our knowledge, there are no studies detecting Babesia species in dogs living in Van province. This study was carried out to investigate the presence and prevalence of babesiosis in dogs in Van province using Polymerase Chain Reaction (PCR) technique. Blood samples were taken from vena cephalica antebrachii in EDTA tubes from a total of 100 randomly selected asymptomatic dogs. DNAs obtained from the samples were investigated by PCR method in which 18S ribosomal RNA gene was amplified for the presence of Babesia spp. Babesia spp. DNA was not found in any of the 100 dogs examined according to PCR results. In conclusion, this study, Babesia spp. species were investigated molecularly for the first time in stray dogs of Van region.