Akademik Veri Yönetim Sistemi
Tezin Türü: Yüksek Lisans
Tezin Yürütüldüğü Kurum: Van Yüzüncü Yıl Üniversitesi, Sağlık Bilimleri Enstitüsü, Viroloji (Veteriner) (Yl) (Tezli), Türkiye
Tezin Onay Tarihi: 2023
Tezin Dili: Türkçe
Öğrenci: FATIMA ABOUNAAJA
Danışman: Ali Rıza Babaoğlu
Özet:
Bovine
viral diarrhea virus (BVDV) infection is detected in cattle populations
worldwide; It is an infection that causes meat, milk, and reproductive
performance losses. Three genotypes of BVDV have been identified worldwide:
BVDV-1, BVDV-2, and HoBi-like pestivirus (BVDV-3). The presence of BVDV
infection in Turkey has been reported since the 1990s, but no mandatory
vaccination program has been implemented so far. The aim of this study is to
determine the genetic diversity of pestiviruses in blood, nasal/vaginal swabs,
and abortion materials taken from cattle in Van, Ağrı, and Iğdır provinces in
the Eastern Anatolia region of Turkey. For this purpose, a total of 110 samples
taken from 80 BVDV-suspected cattle over 6 months of age were tested with one-step
RT-PCR using primers encoding the 5'UTR, autoprotease (Npro), and E2 gene
regions of pestiviruses. As a result of RT-PCR, the pestivirus 5'UTR nucleic
acid positivity rate was 21.81%, the Npro nucleic acid positivity rate was
9.1%, and no positivity for the E2 gene region was detected. In the result of
DNA sequence analysis and phylogenetic analysis of 14 isolates from 12 animals
among the positive amplicons obtained in RT-PCR, all of them were determined to
be of the BVDV-1 genotype. In the phylogenetic analysis of the 5'UTR gene
region of the sequences, BVDV-1e (n = 3), BVDV-1a (n = 1), BVDV-1b (n = 2),
BVDV-1l (n = 1), and BVDV-1d (n = 1) were defined. So phylogenetic analysis
based on the N pro gene region was determined as BVDV-1b (n = 3), BVDV-1a (n =
2), and BVDV-1f (n = 1. In this study, the importance of the role of BVDVs in
genital and respiratory system problems is once again emphasized, and it is
concluded that it should not be ignored in herd screening. The determination of
BVDV genetic diversity in Turkey and worldwide is important in designing and
creating protection-control and eradication strategies, especially in programs
that include vaccination.