Akademik Veri Yönetim Sistemi
23rd meeting of the Balkan Clinical Laboratory Federation (23.BCLF-2015), Sarajevo, Bosna-Hersek, 7 - 09 Ekim 2015, cilt.22, sa.1, ss.23
Background: In this study, effects of
nuclear factor kappa B and formation of
DNA damage on detection of the possible
occurrence of complications depending
on experimental diabetics were
investigated. The evaluation of the
possible role of thymoquinone (TQ) was
aimed in the prevention and treatment of
these complications.
Materials/subjects and methods: For
this purpose, 28 male Wistar-Albino rats
weighing between 200-250 g were used.
Each containing of seven rats, the rats
were divided into four groups of control
(C), TQ (T), diabetes (D) and diabetes +
TQ (DT). TQ was administered as 30
mg/kg/day by oral gavage to the rats in
DT and T groups. In the blood samples
collected after 21 days of trial, the
values of glucose, HbA1c, ALT, AST,
GGT, urea, uric acid, creatinine were
measured as well as the quantities of
NF-KB and 8-OHdG.
Results: It was determined that glucose
levels were increased significantly in D
group (p <0.05), decreased significantly
and approached to control group in DT
(p <0.05) and decreased in the group in
T group was compared to the control
group (p <0.05). HbA1c levels were
significantly increased only in the
diabetic group (p <0.05), and decreased
in DT and approached to the control. It
was observed that ALT and AST activities
were increased significantly in D group
(p <0.05), while significantly decreased
in DT group closing to the control. GGT
activity was the highest in the D group
(p <0.05) but decreased significantly in
DT group compared to D group (p
<0.05). Urea concentrations were the
highest in D and the lowest in T (p
<0.05) while decreased significantly in
DT group compared to the D group (p
<0.05). DNA damage were increased in
both of the diabetics, but, was not
statistically significant. NFκB levels were
the highest in the diabetic group (p
<0.05), while there was no important
difference in TQ and DT groups
compared to the diabetic group.
Conclusions: As a result, it was
observed that increased glucose and
HbA1c levels by STZ-induced diabetes
and indicators of liver and kidney
damages were decreased significantly
and approached to the control group
following the administration of TQ. It was
determined that 8-OHdG which is an
indicator of DNA damage and NFκB
levels were increased in the D group.