SANKO University Innovation in Medicine Summit-3, Gaziantep, Turkey, 11 - 13 May 2017, pp.25
PROFİLE OF URİTİCA DİOİCA L. SEED EXTRACT ON OXİDATİVE DNA DAMAGE İN LİVER TİSSUE AND WHOLE BLOOD OF RADİATİON INDUCED RATS
Tahir ÇAKIR1, Kenan YILDIZHAN1, Zübeyir HUYUT2, Ahmet UYAR3
1 Yuzuncu Yil University, Faculty of Medicine, Department of Biophysics, Van, Turkey
2 Yuzuncu Yil University, Faculty of Medicine, Department of Biochemistry, Van, Turkey
3 Yuzuncu Yil University, Faculty of Veterinary, Deparment of Pathology, Van, Turkey
Aim: Ionizing radiation is commonly used to treatment options in the majority of cancer patients, but radiotherapy applications leads to oxidative DNA damage in non-targeted tissues. In this study, we investigated the radioprotective effects of Uritica dioica L. seed extract (UDSE) against oxidative DNA damage in whole blood and liver tissues of irradiated rats.
Methods: 32 rats (eight weeks) divided into 4 group (n:8). Control group (C); was fed with pellet for 10 days. Radiation group (IR) was fed with pellets for 10 days after exposing 5Gy radiations as a single fraction. Radiation+UDSE (IR+UDSE) group was exposed 5Gy radiations as a single fraction and was fed with UDSE for 10 days. UDSE group (UDSE) was fed with only UDSE for 10 days. The rats were sacrificed under anesthesia (50mg/kg ketamine). 8-hydroxy-2-deoxyguanosine (8-OHdG) and dG levels were measured by high pressure liquid chromatography(HPLC) method in liver tissue and whole blood. Histopathological changes in the liver tissues were determined by immunohistochemistry.
Results: 8-OHdG/106dG levels of liver tissues and whole blood were increased and degenarations were observed in liver tissues in IR group (p?0.05). However, 8-OHdG/106dG levels of liver tissue and whole blood, and degenarative changes of liver tissues were decreased in the IR+UDSE group compared to the IR group (p?0.05). 8-OHdG/106dG levels also were lower in whole bloods of UDSE group, and we did not observe any degeneration in liver tissues of this group.
Conclusions: It was observed that radiation increased oxidative DNA damage and caused to degenerative changes in liver. But, after exposure to radiation, the administration of UDSE that has antioxidant properties may reduce oxidative DNA damage and degenerative changes revealed by the effects of radiotherapy.
Key Words: Antioxidant, Urtica dioica, ionizing radiation, oxidative DNA damage, oxidative stress.