WATER EXTRACT OF HELICHRYSUM PALLASII (SPRENGEL) LEDEB. PREVENTS ROOT DEVELOPMENT OF TOMATO (LYCOPERSICON ESCULENTUM) AND COMMON PURSLANE (PORTULACA OLEARECEA)


Bingöl Ö., Battal A., Erez M. E.

II. International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 1 - 03 September 2020, pp.228

  • Publication Type: Conference Paper / Summary Text
  • City: Edirne
  • Country: Turkey
  • Page Numbers: pp.228
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Weeds compete with the crop plants for available nutrients and moisture in soil, space and light. Therefore, the physiological activities and growth of crops are negatively affected in the presence of weeds. Using of the allelopathic effects of the plant substances is a new strategy to struggle with weeds. The aim of this study was to investigate the alleophatic effect of water extract of Helichrysum pallasii (SPRENGEL) LEDEB. on germination of tomato (Lycopersicon esculentum Mill. cv. S.C. 2121) and common purslane (Portulaca olearecea L. cv. BT Yeşilkulak) plants. Seeds were surface sterillized with 5 % of sodium hypochloride for 10 minutes and washed with sterile distilled water. Additionally, seeds were treated with 70 % of ethanol and washed with sterile distilled water. We tested four different concentrations of Helichrysum pallasii water extract: 5 mg/mL, 10 mg/mL, 20 mg/mL and 40 mg/mL. Sterile distilled water was used as a control application. 10 seeds of tomato and 20 seeds of portulaca were planted in to glass jars including two layers of filter paper. Filter papers were wetted with 5 mL of extracts for each application and sterile distilled water for control. Jars were incubated in controlled growth room (16/8 hours light/dark photoperiod and 25 + 1 °C) for one week. This study was conducted on at least three replicates for each application. Statistical analyses were performed using by GraphPad Prism 8.0.1 package program One -Way Anova analysis. At the end of the study, germination rate was calculated and shoot and root lengths were measured. Germination rate of tomato plant treated with 10 mg/mL, 20 mg/mL and 40 mg/mL extract was significantly lower than control and 5 mg/mL extract. Especially, 40 mg/mL of extract prevented germination of the tomato seeds. The Helichrysum extract did not significantly affect the portulaca germination. According to shoot length results of tomato, 5 mg /mL, 10 mg/mL and 20 mg/mL Helichrysum extract caused importantly decrease in shoot length according to control. Additionally, shoot length of tomato was significantly affected depends on increasing extract concentrations. Similar results were determined for tomato root length. Importantly decrease in shoot length of common purslane was measured in only 20 mg/mL extract application compared to others. The all concentrations of the water extract of Helichrysum negatively affected root length of common purslane according to control. According to our results, the water extract of Helichrysum may have a weed control potential.