Subacute effect of cigarette smoke exposure in rats: Protection by pot marigold (Calendula officinalis L.) extract


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Özkol H., Tülüce Y., Koyuncu I.

TOXICOLOGY AND INDUSTRIAL HEALTH, vol.28, no.1, pp.3-9, 2012 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 28 Issue: 1
  • Publication Date: 2012
  • Doi Number: 10.1177/0748233711401263
  • Journal Name: TOXICOLOGY AND INDUSTRIAL HEALTH
  • Journal Indexes: Science Citation Index Expanded, Scopus
  • Page Numbers: pp.3-9
  • Keywords: Cigarette smoke, pot marigold, oxidative stress, antioxidant, cell injury, OXIDATIVE DAMAGE, DIPHENYL DISELENIDE, IN-VITRO, PLASMA, PHASE, ANTIOXIDANTS, PROTEINS, OXIDANTS, STRESS, MOUSE

Abstract

This study was carried out to determine the preventive effect of Calendula officinalis L. (pot marigold) on rats exposed to cigarette smoke (CS). Rats were divided into three groups as control, CS and CS + pot marigold (PM). The rats in the CS and CS + PM groups were subjected to CS for 1 h twice a day for 23 days. PM (100 mg/kg body weight) was given to rats in the CS + PM group by gavage, 1 h before each administration period. While malondialdehyde, protein carbonyl contents and reduced glutathione level of the CS group increased, their levels diminished by PM administration. In addition, glutathione peroxidase (GPx), superoxide dismutase activities and β-carotene, vitamins A and C levels decreased in the CS group compared to control, however activities of these enzymes and concentration of vitamins were elevated by PM supplementation. This investigation showed that administration of PM supplied relative protection against subacute CS-induced cell injury.

This study was carried out to determine the preventive effect of Calendula officinalis L. (pot marigold) on rats exposed to cigarette smoke (CS). Rats were divided into three groups as control, CS and CS + pot marigold (PM). The rats in the CS and CS + PM groups were subjected to CS for 1 h twice a day for 23 days. PM (100 mg/kg body weight) was given to rats in the CS + PM group by gavage, 1 h before each administration period. While malondialdehyde, protein carbonyl contents and reduced glutathione level of the CS group increased, their levels diminished by PM administration. In addition, glutathione peroxidase (GPx), superoxide dismutase activities and beta-carotene, vitamins A and C levels decreased in the CS group compared to control, however activities of these enzymes and concentration of vitamins were elevated by PM supplementation. This investigation showed that administration of PM supplied relative protection against subacute CS-induced cell injury.