Silibinin and ellagic acid increase the expression of insulin receptor substrate 1 protein in ultraviolet irradiated rat skin.

Gorgisen G., Ozkol H., Tuluce Y., Arslan A., Ecer Y., Keskin S., ...More

Biotechnic & histochemistry : official publication of the Biological Stain Commission, vol.95, no.8, pp.641-646, 2020 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 95 Issue: 8
  • Publication Date: 2020
  • Doi Number: 10.1080/10520295.2020.1753238
  • Journal Name: Biotechnic & histochemistry : official publication of the Biological Stain Commission
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, BIOSIS, Biotechnology Research Abstracts, CAB Abstracts, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Page Numbers: pp.641-646
  • Keywords: Ellagic acid, insulin receptor, IRS1, rat, silibinin, skin, ultraviolet A, ultraviolet B, INDUCED AP-1 ACTIVATION, L. GAERTN. SILYMARIN, SILYBUM-MARIANUM, OXIDATIVE STRESS, SIGNALING PATHWAYS, DIABETES-MELLITUS, RADIATION, KERATINOCYTES, APOPTOSIS, UVA
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Daily exposure to ultraviolet (UV) light induces inflammation and tumorigenesis in the skin. Silibinin and ellagic acid are natural products that exhibit anti-inflammatory and anti-tumorigenic properties. Insulin receptor substrate protein 1 (IRS1) is important for skin homeostasis and physiology, but its activity following UV radiation remains unclear. We investigated the effects of ellagic acid and silibinin on IRS1 expression in ultraviolet A (UVA) and ultraviolet B (UVB) irradiated rat skin. Forty-two female Wistar rats were divided randomly into six groups of seven animals. The dorsal skin of rats was exposed to UVA + UVB, then treated with ellagic acid and silibinin by gavage. IRS1 expression in skin tissues was determined by western blot analysis. IRS1 expression increased significantly following treatment with ellagic acid and silibinin in UVA + UVB irradiated skin compared to the UVA + UVB only group. After UVA + UVB treatment, ellagic acid effected greater induction of IRS1 expression than silibinin. Our findings suggest that the photoprotective roles of ellagic acid and silibinin may be due to induction of IRS1 expression in UVA + UVB treated rat skin.

Keywords: Ellagic acid; IRS1; insulin receptor; rat; silibinin; skin; ultraviolet A; ultraviolet B.