EVALUATION THE CYTOTOXIC EFFECTS OF DIFFERENT ORAL ANTISEPTICS ON PRIMARY GINGIVAL FIBROBLASTS


Alpaslan Yaylı N. Z., Dikilitaş A., Keskin Tunç S., Ünalan Değirmenci B., Taşpınar M.

23rd BaSS CongressDental Medicine of the Younger vs. Elderly Patients, Yaş, Romania, 10 - 12 May 2018, pp.251

  • Publication Type: Conference Paper / Summary Text
  • City: Yaş
  • Country: Romania
  • Page Numbers: pp.251
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Background: It was aimed to investigate time and dose-dependent possible cytotoxic
effects of different oral antiseptics on the primary gingival fibroblasts. Methods: In this study,
gingival fibroblast cells were obtained by primary cell culture techniques from a patient who had
indication for orthodontic tooth extraction. Three antiseptics which contain chlorhexidine
digluconate, benzydamine hydrochloride and povidone iodine were prepared as 0,5%, 2,5%, 5%,
12,5%, 25%, 50% and 100% concentrations and these concentrated antiseptics were applied to the
cells for 0, 30, 60 and 180 sec. At the end of the incubation, XTT cytotoxicity test was performed.
The Graphad Prism program was used to assess the absorbances that obtained and software
SPSS 22.0 was used for the statistical analysis. Results: In all antiseptics applied groups, when the
antiseptic concentration and application time increased, cell death was increased comparing to the
control group. For the 30 seconds applications of 0,5% concantrated antiseptics, there was no live
cell number difference comparing to the control group (p>0,05). Also statistically significant live cell
number decrease was observed after the all application times of %2,5, %12,5, %25, %50 and %100
concantrated antiseptics (p<0,05). However, when time and dose-dependent effects were evaluated
for all three antiseptics, chlorhexidine digluconate containing solution was the most cytotoxic
antiseptic on gingival fibroblast cells. Conclusions: Based on this study results, we concluded that
antiseptics contents could cause different cell death rates. Application times, concentrations and
contents could be important for the clinical usage of oral antiseptics.