Determination of cytotoxic, genotoxic, and oxidative damage from deltamethrin on primary hepatocyte culture of Lake Van fish,Alburnus tarichi

Kiraccakali A. N., Oğuz A. R.

CHEMISTRY AND ECOLOGY, cilt.36, sa.7, ss.651-662, 2020 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 36 Sayı: 7
  • Basım Tarihi: 2020
  • Doi Numarası: 10.1080/02757540.2020.1781098
  • Dergi Adı: CHEMISTRY AND ECOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Agricultural & Environmental Science Database, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Food Science & Technology Abstracts, Geobase, Pollution Abstracts, Veterinary Science Database
  • Sayfa Sayıları: ss.651-662
  • Anahtar Kelimeler: Deltamethrin, liver, Primer hepatocte culture, Lake Van fish, FRESH-WATER FISH, LIPID-PEROXIDATION, CYPRINUS-CARPIO, DIFFERENTIAL TOXICITY, EASTERN ANATOLIA, TRANS-PERMETHRIN, RAINBOW-TROUT, NILE TILAPIA, STRESS, EXPOSURE
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Deltamethrin is a widely used pyrethroits worldwide. Although the chemical is used to combat insects, it has effects on other non-target organisms. Deltamethrin is extensively used in agriculture, animal husbandry, and domestic areas in the Lake Van basin. The aim of this study was to determine the cytotoxic, genotoxic, and oxidative damage from deltamethrin on the primary hepatocyte culture of Lake Van fish (Alburnus tarichi). In this study, the toxic effects of different concentrations (0.01, 0.1, 1, and 10 mu M) of deltamethrin in the primary hepatocyte culture of Lake Van fish were investigated via liver enzymes aspartase aminotransferase (AST) and alanine aminotransferase (ALT), and the total antioxidant status (TAS), total oxidant status (TOS), lipid peroxidation (MDA), and DNA damage (8-OHdG). Deltamethrin caused an increase in the AST and ALT levels dependent on the dosage and time. The TAS and TOS levels increased at the end of 24 h and there was no difference at the end of 48 h. Deltamethrin did not affect the MDA level, but increased the 8-OHdG (P < 0.05). In conclusion, it can be said that high doses of deltamethrin (1 and 10 mu M) have a toxic effect on the primary hepatocyte culture of Lake Van fish.