Detection of Se, Vit. E, Vit. A, MDA, 8-OHdG, and CoQ10 Levels and Histopathological Changes in Heart Tissue in Sheep with White Muscle Disease

YILDIRIM S., OZKAN C., Huyut Z., Cinar A.

BIOLOGICAL TRACE ELEMENT RESEARCH, vol.188, no.2, pp.419-423, 2019 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 188 Issue: 2
  • Publication Date: 2019
  • Doi Number: 10.1007/s12011-018-1434-7
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.419-423
  • Keywords: 8-OHdG, CoQ10, Histopathological findings, Sheep, White muscle disease, SELENIUM DEFICIENCY, OXIDATIVE STRESS, NITRIC-OXIDE, TROPONIN-I, COENZYME-Q10, HPLC, DNA, METAANALYSIS, LAMBS
  • Van Yüzüncü Yıl University Affiliated: Yes


This study was carried out to determine vit. E, Se, vit. A, malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and ubiquinone-10 (CoQ10) levels and histopathological changes in sheep with white muscle disease (WMD). A total of 30 sheep were used; 20 sheep with WMD were brought to our clinic for diagnosis and treatment at various times, and 10 healthy sheep were in the control group. The Se, vit. E, vit. A, MDA, 8-OHdG, and CoQ10 values of the healthy and WMD sheep were as follows: 0.917 +/- 0.037, 0.790 +/- 0.067; 1.190 +/- 0.011, 1.090 +/- 0.021; 5.400 +/- 0.275, 5.200 +/- 0.173; 1.602 +/- 0.264, 2.636 +/- 0.576; 0.656 +/- 0.197, 1.485 +/- 0.271; and 0.280 +/- 0.044, 1.753 +/- 0.551 respectively (p<0.05). According to histopathological and immunohistochemical findings in the WMD group, hyaline degeneration, Zenker's necrosis, and dystrophic calcification were observed in the muscle fibers. Immunohistochemically, 8-OHdG staining of the heart tissue determined a severe 8-OHdG expression in the WMD group. The findings of this study suggest that MDA, 8-OHdG, and CoQ10 values could be used as diagnostic and prognostic biomarkers in sheep affected with WMD.