Akademik Veri Yönetim Sistemi
1. Euroasia Biochemical Approaches & Technologies Congress (1.EBAT-2018), Antalya, Türkiye, 27 - 30 Ekim 2018, cilt.1, sa.1, ss.55
Aim: This research was planned to determine the various concentrations of the Diplotaenia Turcica
extract,which is traditionally known to be good for diabetes, blood pressure, and rheumatism, to be
used in several studies in cell culture.
Material and Method: The NRK-52E cells were proliferated in an environment of 37oC, 5% CO2 and
95% of humidity, and in a RPMI 1640 DMEM media containing 1% penicillin/streptomycin 2 mM LGlutamine and 10% FBS. The cells were cultured in a plate of 96 as to be 104
. Diplotaenia Turcica
plant was prepared with ethyl alcohol and water extract. The prepared Diplotaenia Turcica plant was
applied to the renal cell line for 6,12 and 24 hours of time. Afterwards, the MTT assay was performed
and liveliness percentage was determined.
Findings: Different concentrations of Diplotaenia Turcica extract applied in the 6,12 and 24th hours
had no effects on the cell liveliness under 50 µg/ml of concentrations and similar results to the control
group was observed. 50 µg/ml concentration was observed to increase the cell proliferation in the 3rd
hour by 20%, 40% in the 6th hour, and by the 24th hour resulted in a liveliness rate close to the
control group and did not change the proliferation. But, all concentrations above 50 µg/ml were
determined to cause cell death in all application periods and that these concentrations are cytotoxic.
Result: It was found that concentrations over 50 µg/ml of Diplotaenia Turcica in future in vitro studies
cannot be used as a useful dose for being cytotoxic but, 50 µg/ml and below are useful
concentrations.
Keywords: Diplotaenia Turcica, NRK-52E Cell, MTT