The effect of thymoquinone at proliferative and toxic doses on Ripk1 gene expression in kidney cells

Usta A., Yüksek V., Dede S.

2. International Congress on Biological and Health Sciences (2.ICBH-2022), Afyonkarahisar, Türkiye, 24 - 27 Şubat 2022, cilt.1, sa.97, ss.127

  • Yayın Türü: Bildiri / Özet Bildiri
  • Cilt numarası: 1
  • Basıldığı Şehir: Afyonkarahisar
  • Basıldığı Ülke: Türkiye
  • Sayfa Sayıları: ss.127
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

The study focused on thymoquinone, one of the main terpenes known as black cumin essential oil. The possibility that TQ may be harmful as well as beneficial on healthy kidney cells has been investigated. We investigated the effect of thymoquinone on necrosis at beneficial and toxic doses. For this purpose, NRK-52E rat kidney epithelial cell line was used as material in the study. NRK-52E cells were proliferated by systematically passaged in an appropriate medium under in vitro conditions. Proliferative and toxic doses were determined according to the results of the MTT cell viability test performed to determine the proliferative (TQp-10µM) and toxic (TQIC50-60µM) values of TQ at the 24th and 48th hours. It consists of 6 groups as 24th hour control, TQp, TQIC50 and 48th hour control, TQp, TQIC50 groups. Ripk1 gene expressions from necrotic genes were measured by RT-qPCR in obtained RNA samples at 24 and 48 hours of these doses. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control gene in PCR. The fold change was found using the formula 2-ΔΔCt. According to the results of this analysis; It was determined that high dose TQ-induced necrosis showed activity in NRK-52E kidney cell line. There was a slight increase in Ripk1 gene expression in the 24th hour TQIC50 group and a higher increase at 48th hours. Expression was found to be low in all other groups. As a result, it was concluded that TQ was safe at proliferative concentration at 24 and 48 hours.