Akademik Veri Yönetim Sistemi
International Biochemistry Congress – 2017 /28 th National Biochemistry Congress (TurkJBiochem-2017), Erzurum, Türkiye, 19 - 23 Eylül 2017, cilt.42, sa.1, ss.35
OBJECTIVE: The present study was planned to investigate possible
prooxidant/antioxidant mechanisms and determining the effect on cell viability in
bone cell lines involved in fluoride metabolism and the role of certain vitamins(A,
D, E and C).
MATERIALS -METODS : Cells were replicated in vitro with two to three regular
passages per week. NaF IC50 and vitamin doses were determined by MTT. Cells
were seeded 104 to 96-well culture plates and 106 to flasks . The groups were
determined as control group, NaF, vitamin and NaF+vitamin groups. The cells were
harvested by trypsinization following 24 -hour incubation and samples were
prepared by disintegration by freeze-thaw method for TAS and TOS analysis. It was
used MTT test for the effect of some vitamins on various doses of NaF and
commercial kit for TAS and TOS.
RESULTS: TAS levels were significantly decreased in NaF group (p≤0.05), while
TAS levels were close to control except vitamin C. It was found that TOS levels
increased significantly in the NaF-treated group (p≤0.05), but in all groups vitamin+NaF decreased (p≤0.05). It was detected that the OSI was the highest in the NaF group, in groups given vitamin were close to control except NaF+
vitamin C.
CONCLUSION: it was determined that NaF administration in the osteoblastic
cell line increased oxidative stress and decreased following vitamin application. It
has been concluded that the cell viability of osteoblastic cell line is compatible
with the oxidative balance and that the change of oxidative balance in cell death
due to NaF application is effective.
Keywords: Antioxidant, Cell culture, NaF, Vitamins