DETERMINATION OF THE OCCURRENCE FREQUENCY OF CRYPTOSPORIDIUM SP. IN CHILDREN BROUGHT TO THE HOSPITAL WITH THE COMPLAINT OF DIARRHEA USING DIFFERENT METHODS


Ekici A., Unlu A. H. , Halidi A. G. , Aydemir S., Yilmaz H.

APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH, vol.19, no.4, pp.2905-2913, 2021 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 19 Issue: 4
  • Publication Date: 2021
  • Doi Number: 10.15666/aeer/1904_29052913
  • Journal Name: APPLIED ECOLOGY AND ENVIRONMENTAL RESEARCH
  • Journal Indexes: Science Citation Index Expanded, Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Environment Index, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.2905-2913
  • Keywords: cryptosporidiosis, children, diarrhea, immunochromatographic test, nested PCR, SPP.

Abstract

Cryptosporidium is a parasite responsible for diarrhea in humans. Practitioners rarely routinely request Cryptosporidium diagnostic tests; thus, its prevalence is likely underrated. The prevalence of cryptosporidiosis among children brought to the hospital with the complaint of diarrhea was investigated using 3 different methods, comprising nested PCR, immunochromatographic testing, and microscopic examination with modified acid-fast staining. Cryptosporidium sp. was detected in 42/150 children with diarrhea (28%) and in 1/50 children in the control group (2%). The main complaint by children admitted to hospital with Cryptosporidium was diarrhea. Additionally, some clinical symptoms/signs like abdominal pain, nausea, vomiting, stool mucus, weakness, weight loss, and anorexia were correlated with cryptosporidiosis. Results from the 3 methods were compared and nested PCR and immunochromatographic testing were the most reliable. Among other pathogens and parasites found in stool samples, Cryptosporidium is a significant cause of hospitalization due to gastrointestinal disease in children in Van, Turkey.