Detection of arbuscular mycorrhizal fungi within colonised roots of the Gramineae family members by nested-polymerase chain reaction (PCR)

Demir S., SIPAHIOGLU H. M., Kaya İ., Usta M., SAVUR O. B.

Archives of Phytopathology and Plant Protection, cilt.44, sa.11, ss.1061-1067, 2011 (Scopus) identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 44 Sayı: 11
  • Basım Tarihi: 2011
  • Doi Numarası: 10.1080/03235401003755221
  • Dergi Adı: Archives of Phytopathology and Plant Protection
  • Derginin Tarandığı İndeksler: Scopus
  • Sayfa Sayıları: ss.1061-1067
  • Anahtar Kelimeler: arbuscular mycorrhizal fungi, Gramineae, nested-PCR
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

In this study, it is aimed to asses the association of arbuscular mycorrhizal (AM) fungi within colonised rhizosphere of Gramineae family members through a survey by using nested polymerase chain reaction method in Van province (Turkey). From 24 agro-ecological fields, a total of 82 samples belonging to Gramineae family were tested by molecular methods. The presence of Glomus intraradices and Glomus mosseae was ascertained in 10 plants belonging to eight different species by using fungus specific primers. Root colonisation ranged from 6 to 37% within rhizosphere of Gramineae family members and the average root colonisation by AM fungi was 22%. © 2011 Copyright Taylor and Francis Group, LLC.