Hematological and Hepatic Effects of Ulexite in Zebrafish


ALAK G., ÖZGERİŞ F. B. , Yeltekin A. Ç. , PARLAK V., UÇAR A., ÇAĞLAR Ö., ...More

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, vol.80, 2020 (Peer-Reviewed Journal) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 80
  • Publication Date: 2020
  • Doi Number: 10.1016/j.etap.2020.103496
  • Journal Name: ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Agricultural & Environmental Science Database, BIOSIS, CAB Abstracts, EMBASE, Environment Index, Greenfile, MEDLINE, Pollution Abstracts, Veterinary Science Database
  • Keywords: Ulexite, boron, zebrafish, nuclear abnormality, micronucleus frequency, DNA damage, apoptosis, liver, blood, MICRONUCLEUS FREQUENCY, NUCLEAR ABNORMALITIES, PERIPHERAL-BLOOD, OXIDATIVE STRESS, BORON-COMPOUNDS, BORIC-ACID, TROUT, 8-HYDROXY-2-DEOXYGUANOSINE, MYELOPEROXIDASE, INFLAMMATION

Abstract

The ulexite (UX), a borate mineral, is used as boron source and commonly used in various industrial processes. The hematological and hepatic effects of UX were investigated by exposing adult zebrafish to UX (5, 10, 20 and 40 mg/L) over 96 hours. The blood and liver tissues were taken at the end of the trial period then micronucleus (MN) rates, oxidative DNA damage (8-OHdG), apoptosis (Caspase-3), superoxide dismutase (SOD), catalase (CAT), glutathione pemxidase (GPx), myelopemxidase (MPO), paraoxonase (PON), arylesterase (AR) and lipid peroxidation (MDA) levels were determined. Genotoxic damage by UX occurred only at 40 mg/L in the blood MN assay. Oxidative stress, oxidative DNA damage and apoptosis in liver also occurred at this dose. Moreover, 5-20 mg/L doses led to decreases of DNA damage and apoptosis levels via promoting antioxidant system in liver tissues. UX exhibits beneficial roles on blood and liver tissues of zebrafish at relatively lower doses, which may be relevant to nutritional and medicinal industries.