ISPEC 10th International Conference On Engineering & Natural Sciences , Siirt, Turkey, 16 - 18 May 2021, pp.463
TOTAL PHENOLIC AND FLAVONOID CONTENTS OF Malus sylvestris L. EXTRACTS
Malus sylvestris L. EKSTRELERİNİN TOTAL FENOLİK VE FLAVONOİD İÇERİĞİ
Neşe ERAY VURAN
Res. Asist., Van Yüzüncü Yıl University, Faculty of Science, Department of Molecular Biology and Genetic, email@example.com (Corresponding Author), Orcid ID: 0000-0001-6387-1493
Deniz İRTEM KARTAL
Assist. Prof. Dr., Van Yüzüncü Yıl University, Faculty of Science, Department of Molecular Biology and Genetic, firstname.lastname@example.org, Orcid ID: 0000-0001-9669-5828
Prof. Dr., Van Yüzüncü Yıl University, Faculty of Science, Department of Molecular Biology and Genetic, email@example.com, Orcid ID: 0000-0003-2199-6348
Malus sylvestris L. (Wild Apple) is a small fruit tree belonging to the Rosaceae family. Given their high light demands and low competitive abilities, wild apple is naturally scattered in forest gaps and forest margins. The aim of the study is to reveal the total phenol and flavonoid content and to demonstrate a possible biochemical difference between water and ethanolic extracts of the plant. For this purpose, total phenolic (TPC) and ﬂavonoid (TFC) contents in ethanolic and water extracts of fruit and seed were determined.
The highest extraction yield was obtained from the ethanolic extracts of the fruit. The percent yield of extraction of the fruit with ethanol was found as 10,57 % (w/w) with water as 10, 06 % (w/w). The highest total phenol content was obtained from the seed ethanol extracts and calculated as 413,7±1,11 µg GAE/mg (p<0.0001). The fruit ethanol extract was also found to have high total phenolic content. In both Quercetin and Catechin standards, total flavonoid contents of the fruit ethanol extract was found to be higher than the other organs. The amount of total flavonoids of ethanolic extracts of fruit were determined as 554,6±1,83 QE/ mg dry extracts and 491,7±2,54 µg CE/ mg dry extracts, respectively (p<0,0001). The fruit water and seed ethanol extracts were also found to have high total flavonoid content.
As a result, the fruit extracts of Malus sylvestris L. were more successful than other organs in terms of total flavonoid content. The highest total phenol content was obtained from the seed ethanol extracts. Due to the higher solubility of the compounds in alcohol, the total phenol and flavonoid contents of ethanol extracts of the plant were found to be higher than the water extracts.
Keywords: Flavonoid, Malus sylvestris L., Phenolic
Acknowledgment: This work was supported by the Scientific Research Projects Unit of Van Yüzüncü Yıl University (project no: TDK-2019-8379).