Akademik Veri Yönetim Sistemi
11th INTERNATIONAL CONFERENCE on AGRICULTURE, ANIMAL SCIENCES and RURAL DEVELOPMENT, Muş, Türkiye, 3 - 05 Mart 2023, cilt.1, sa.1, ss.4-5
In this study, the alternative use of natural extract obtained from the tree root, for which patent application has been filed, was evaluated in terms of fish health. The components of the extract were analysed. Phenotypic, biochemical and molecular identifications of Lactococcus garvieae and Staphylococcus epidermidis agents used in the study were performed. The minimum inhibition concentration values were determined by the MIC test of the extract. In the antibiogram test, the extract was compared with the antibiotics Florfenicol and Ampicillin. As a result of the study, it was seen that the results of the biochemical catalase oxidase and gram staining of the bacteria used were the results of the bacteria. In the MIC test results of the extract, it was determined that bacteria did not develop in the 25, 5 and 1 dilutions. According to the dendrogram obtained from the sequence results of the bacteria; It was observed that the L. garvieae isolate used in the study was 99.8% similar according to the closest 5 results as a result of blasting. It was observed that S. epidermidis isolate was 99.3% similar according to the closest 5 results as a result of blasting. As a result of the antibiogram test performed to test the usability of the extract used in the study as an antibiotic alternative; It was observed that the extract had the same effect as Ampicillin on L. garvieae and was 4% more effective than Florfenicol antibiotic. It was observed that the extract was 83% more effective than Ampicillin and Florfenicol antibiotics according to the S. epidermidis agent.The extract used in the study was tested on bacterial agents in seafood and successful results were obtained. We think that the substance will be useful in different fields after it is turned into a product and the necessary permits are obtained.