Phylogenetic diversity of Nocardia sp. obtained from different water environments


Seçkin H., Önalan Ş.

Research Journal of Biological Science, cilt.13, sa.2, ss.37-44, 2020 (Hakemli Dergi)

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 13 Sayı: 2
  • Basım Tarihi: 2020
  • Dergi Adı: Research Journal of Biological Science
  • Derginin Tarandığı İndeksler: Other Indexes
  • Sayfa Sayıları: ss.37-44
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

The aim of this study was to investigate the isolation and typing of Nocardia species, which

are found in many water resources and have microbial importance. Agent isolations were carried out from

sediment samples taken from different points determined around Iğdır Fish Lake. DNA isolations were

carried out from the isolated Nocardia species. Subsequently, 16S rRNA gene region sequence analyzes

were performed. Sequence analyzes for each isolated bacteria were aligned and these results determined

similarities and differences at the level of group and genus of different origins and individual populations.

As a result of the study, 2 different types of bacteria were isolated. In the GenBank database, the isolated

bacteria were found to have 99.78% and 99.30% similarity with the species with 99% and higher sequence

matching. Nucleotide sequencing differences were found to be 0.3% with reported to be isolated from

different water sources. Different studies are needed to determine the differences at the gene level of the

same type of bacteria isolated from different sources, and to determine the pathogenicity and virulence

characteristics among the isolates.