Investigation of biochemical and histopathological effects of vitamin c, selenium and therapeuthical ultrasound application on muscular injury formed in rats


TAT N. M. , TÜLÜCE Y., KARA M., TAT A. M. , ÖZKOL H.

International Congress on Advances in BioScience and Biotechnology, Sarajevo, 25 - 27 October 2017

  • Publication Type: Conference Paper / Summary Text

Abstract

INTRODUCTION: Muscle injury is the type of soft tissue injury and then occured increased oxidative damage . PURPOSE: The efficiency of therapeutic ultrasound treatment and various antioxidant substances was compared in experimental muscular injury. Some serum enzymes, oxidative stress biomarkers, myeloperoxidase (MPO) activity, inflammation markers interleukin-6 (IL-6) and tumor necrosis factor-a (TNF- a) were evaluated histopathological examination. METHODS: Each group enclosing 6 rats were constituted to six groups control, only injury (OI), ultrasound (U), vitamin (Vit C), selenium (S) and mix (M). The muscle injury was formed through laceration of gastrocnemius muscle. Treatment was applied three times to groups. Therapeutic ultrasound, vitamin C, selenium and combination of them was applied respectively to the rats. Rats were sacrificed in the sixth day. Blood and tissue samples were obtained for biochemical and histological analyses. RESULTS: While OI group compared to control in terms of serum enzymes, significant (p<0.05) elevations of CK, AST and ALT as well as slight increase of LDH. CK, ALT and AST levels reversed to control values in almost all treatment groups (p<0.05) as well as slight decrease of LDH. The shifts in total antioxidant level were insignificant. Total oxidant status (TOS) and oxidative stress index (OSİ) increased in OI group and it decreased in S and M. However; among these alterations only TOS in muscle tissue was found to be statistically significant (p<0.05). While MPO activity increased in OI group, it decreased other treatment groups. However only the increase on MPO in the blood tissues of OI group and the decrease in Vit C group were statistically significant (p<0.05). There were not any marked alteration between groups in terms of serum inflammation markers (IL-6 and TNF-a) and histological findings. CONCLUSION: Results of this study revealed that apart from therapeutic ultrasound treatment, supplementation of vitamin C and selenium may also be useful treatment of muscular injury. However further studies are needed to support these results.