The 26th Meeting of the Balkan Clinical Laboratory Federation, Skopje, Macedonia, 3 - 05 October 2018, vol.26, no.18, pp.93
In this study, it was aimed to investigate the presence and the prevalence of Enterococcus faecalis and Enterococcus faecium strains isolated from the urine and stool samples. A total of 500 routine urine and feces samples were used for testing as the study materials, and a total of 349 Enterococcus spp. were collected for investigation. For the isolation, blood agar and bile esculin agar were used. DNA isolations of the 24-hour growth cultures of possible enterococci were carried out using a DNA isolation kit. Out of 350 routine urine and 150 stool samples taken with the approval of the patients, 235 (67.1%) and 114 (76%) Enterococcus spp. were isolated respectively. Using the multiplex PCR method with species specific primers, 136 (57.8%) of urine and 22 (19.2%) of stool originated enterococcal strains were identified as Enterococcus faecalis; on the other hand, 17 (7.2%) of urine and 61 (53.5%) of stool originated enterococci were identified as Enterococcus faecium. As a result of the study in Van, Turkey, the isolation rate of Enterococcus faecalis and Enterococcus faecium strains were found to be lower than other regions.