Effects of Leontice leontopetalum and Bongardia chrysogonum on oxidative stress and neuroprotection in PTZ kindling epilepsy in rats

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Erkeç Ö. E., Arihan O., Kara M., Karatas E., Erten R., Demir H., ...Daha Fazla

CELLULAR AND MOLECULAR BIOLOGY, cilt.64, sa.15, ss.71-77, 2018 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 64 Sayı: 15
  • Basım Tarihi: 2018
  • Doi Numarası: 10.14715/cmb/2017.64.15.12
  • Dergi Adı: CELLULAR AND MOLECULAR BIOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.71-77
  • Anahtar Kelimeler: Bcl-2, Brain, Bongardia chrysogonum, Cyclin-B1, GABA(A), kindling model, Leontice leontopetalum, Oxidative stress, Pentylenetetrazol, HIPPOCAMPAL-NEURONS, INDUCED SEIZURES, NIGELLA-SATIVA, ANTIOXIDANT, RECEPTOR, EXPRESSION, EXTRACT, CORTEX, APOPTOSIS, DENSITY
  • Van Yüzüncü Yıl Üniversitesi Adresli: Evet

Özet

Abstract: We investigated the effects of Leontice leontopetalum and Bongardia chrysogonum on apoptosis, gamma-aminobutyric acid (GABAA) receptor positive cell number, cyclin-B1 and bcl-2 levels and oxidative stress in pentylenetetrazol (PTZ) kindling in rats. Kindling was produced by subconvulsant doses of PTZ treatments in rats; PTZ treated (PTZ), PTZ+L. leontopetalum extract treated (PTZ+LLE) and PTZ+B. chrysogonum extract treated (PTZ+BCE) groups. Extracts were given a dose (200 mg/kg) 2h before each PTZ injection. PTZ treatment significantly decreased the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and bcl-2 levels and increased the total oxidant status (TOS), malondialdehyde (MDA), cyclin B1, oxidative stress index (OSI) and number of neurons that expressed GABAA receptors when compared to the control. LLE and BCE possessed antioxidant activity in the brain and ameliorated PTZ induced oxidative stress, decreased cyclin-B1, increased bcl-2 levels, and kept the GABAA receptor number similar to that of the control despite the PTZ application.
Key words: Bcl-2; Brain; Bongardia chrysogonum; Cyclin-B1; GABAA; kindling model; Leontice leontopetalum; Oxidative stress; Pentylenetetrazol.

Abstract: We investigated the effects of Leontice leontopetalum and Bongardia chrysogonum on apoptosis, gamma-aminobutyric acid (GABAA) receptor positive cell number, cyclin-B1 and bcl-2 levels and oxidative stress in pentylenetetrazol (PTZ) kindling in rats. Kindling was produced by subconvulsant doses of PTZ treatments in rats; PTZ treated (PTZ), PTZ+L. leontopetalum extract treated (PTZ+LLE) and PTZ+B. chrysogonum extract treated (PTZ+BCE) groups. Extracts were given a dose (200 mg/kg) 2h before each PTZ injection. PTZ treatment significantly decreased the glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activities and bcl-2 levels and increased the total oxidant status (TOS), malondialdehyde (MDA), cyclin B1, oxidative stress index (OSI) and number of neurons that expressed GABAA receptors when compared to the control. LLE and BCE possessed antioxidant activity in the brain and ameliorated PTZ induced oxidative stress, decreased cyclin-B1, increased bcl-2 levels, and kept the GABAA receptor number similar to that of the control despite the PTZ application.
Key words: Bcl-2; Brain; Bongardia chrysogonum; Cyclin-B1; GABAA; kindling model; Leontice leontopetalum; Oxidative stress; Pentylenetetrazol.