Akademik Veri Yönetim Sistemi
The International Conference on Agriculture, Forest, Food Sciences and Technologies(ICAFOF), Nevşehir, Türkiye, 15 - 17 Mayıs 2017, ss.1167
Broccoli (Brassica oleracea Italica) plants with symptoms of leaf curling, enation, fasciation, dwarfing, vain banding, swelling and cracking symptoms were observed in greenhouse grown plants in Malatya province in 2016. Genomic DNA isolation was made from leaf tissue and midrib of symptomatic and non-symptomatic plants. By using R16mF2/R16mR1 and R16F2n/R16R2 primers 16S rDNA fragments were subjected to nested polymerase chain reaction (Nested-PCR) for the involvement of possible phytoplasma infection. Symptomatic plants were yielded approx. 1.2-kb DNA fragments. Amplified PCR fragments were cloned into a proper plasmid vector and transformed into competent Escherichia coli strain JM 109. Recombinant plasmid DNA was isolated and sequenced bi-directionally. The presence of white fly was observed on and around the symptomatic plants. Fifteen white flies were sampled from greenhouse to assess the presence of the suspected agent. The genomic DNA was isolated and subjected to Nested-PCR by using same primer pairs. None of the tested white fly was reacted positive for the presence of expected DNA fragments. Based on sequence data, phylogeny analysis and virtual restriction fragment length polymorphism (RFLP) analysis, an unidentified and uncultured bacterium has been reported in broccoli plants in Malatya. The attempts to visualize the agent by electron microscopy in isolated host tissues are still in progress.