In Vitro Cytotoxic Effects and Quality Control Studies of Black Cumin Oils and Soft Capsules in the Turkey Market


Üstündağ B., Derici Eker E., Şahin N. Ö.

International Agricultural, Biological & Life Science Conference, Edirne, Turkey, 2 - 05 September 2018, pp.799

  • Publication Type: Conference Paper / Summary Text
  • City: Edirne
  • Country: Turkey
  • Page Numbers: pp.799
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

Nowadays, natural products are frequently used in the preparation of food additives, cosmetics and drug preparations. Therefore many medically effective plant species have had a significant share of steadily increasing trends in the world market. Black cumin (Nigella sativa) has been used for many years in the Middle East, Asia and Africa, and in recent years in Europe, it has been used for the purpose of preventing diseases from being treated, promoting healthy life and increasing the quality of life. Nigella sativa (NS) is a member of the Ranunculaceae family and is also called black seed, black caraway or fertility grain. Considering the potential medicinal properties of thymoquinone, one of the most important components of black seed oil, it has been chosen as a traditional healing resource. In this study, the Turkey market, by providing the black seed oils and soft capsules with different formulations which are marketed by several manufacturers quality control tested and thus are intended to be compared in terms of efficacy and safety. In this context; GC analysis and HPLC analysis were performed to determine the amount of fatty acids components and ratios in the content of black cumin oil, and to determine the amount of thymoquinone, which is a major component of black cumin oil. In addition, MTT test was performed by applying the capsules and oils forms obtained from the market to the MCF-7 breast cancer cell line in order to examine the in vitro cytotoxic effect of the black cumin. The composition of fatty acid methyl esters of soft capsule forms and oils in the market of Nigella sativa preparations is shown by GC analysis. The most common unsaturated fatty acids in fat and soft capsule preparations are linoleic acid (~ 56.55%) and oleic acid (~ 25.30%). The most saturated fatty acid is palmitic acid (~ 11.5%). The soft capsules are compared among themselves; the YK1 soft capsule has lower proportions than the YK2 and YK3 preparations in terms of both saturated and unsaturated fatty acids. Nigella sativa soft capsules and oils obtained from the market were also investigated by HPLC method with methanol extract prepared or not containing thymoquinone. The highest amount of thymoquinone was found in Y2 oil (2.9728 mg/ml), while the group with the least amount of thymoquinone was YK1 soft capsule group (0.8917 mg/ml). Cell viability was assessed by MTT assay compared to negative control. There was no statistically significant difference in IC50 between two groups (capsule[1]oil) (p>0.05) (0,123). 0.5% DMSO was used as a negative control and 10 μM thymoquinone was used as a positive control. As a result of the MTT analysis, it was observed that the Y2 test group had the highest cytotoxic effect at 90.21% compared to the IC50 values calculated from the absorbance values obtained at 48 hours from each test group. The least cytotoxic group was YK1 with 94.98%. These ratios are in parallel with our HPLC analysis. The result of HPLC analysis and MTT analysis support each other. As a result, in the light of previous studies, our thinking about the cytotoxic effect of N. sativa in the MCF-7 cell culture medium has become more grown. As supported by many studies, the cytotoxic effect of black cumin may suggest a particularly promising new treatment method and the development of a drug against cancer. In addition, our study is an in vitro cell-based study and should be supported by in vivo studies.

Keywords : Black cumin, Cytotoxic effects, Cell culture, Quality control