Akademik Veri Yönetim Sistemi
13th International Symposıum on Pharmaceutical Sciences, Ankara, Türkiye, 22 - 25 Haziran 2021, ss.120-121
Introduction: Origanum
minutiflorum belongig to the Lamiaceae used as ‘Oregano’ in Turkey. O. minutiflorum
is an endemic species that has been sustainably collected for years from
Sütçüler, province of Isparta in Turkey. Although O. minutiflorum is among
the commercial Oregano and is widely used with various nutrients in Turkey and
in many exporting countries, there is not enough studies on its pharmacological
effects (1). LOX enzymes are associated with inflammation-related diseases, and
neurodegenerative diseases (2). In this study is aimed to determination of in vitro lipoxygenase enzyme inhibition
of the aerial parts of O. minutiflorum.
Materials and Methods: Plant material was collected from
Sütçüler, Isparta, Turkey in 2017. Crude extract was prepared with 70% ethanol
and was subjected to liquid-liquid extraction according to polarity of the
following solvents; n-hexane,
dichloromethane, etylacetate and n-butanol,
resprectively. Llipoxygenase (LOX) inhibition activity was made
spectrophotometrically by modifying the method defined by Baylac and Racine (3).
Nordihydroguyaretic acid (NDGA) was used as positive control. All measurements
were repeated three times and the results were expressed as Mean ± Standard
deviation.
Results: It was determined that the
dichloromethan extract (100 μg/mL) showed the highest inhibition on the LOX
enzyme (82.33±0.94%), while % Inhibition of the other extracts (100 μg/mL),
40.94±1.81% (ethyl acetate), 24.50±0.50% (n-buthanol) and 17.45±0.55% (hexan).
No activity found in the residual extract. NDGA was determined as 99.00±0.00
µg/mL.
Conclusions: As far as we know, the present work is the
first contribution into LOX enzyme inhibition activity of O. minutiflorum.
Acknowledgements
We are
grateful to Prof. Dr. Yavuz Bulent Kose, Faculty of Pharmacy, Department of Pharmaceutical
Botany, Anadolu University, Eskişehir, Turkey, for determination of the plant
specimen.
References: 1. Baser, K. H. C. (2002). The Turkish
Origanum species. In: Oregano, The genera Origanum and Lippia, Ed.:
S.E.Kintzios, Taylor and Francis, London, pp. 109-126.
2. Sacan, O., Turhan, E. Y. (2014).
Lipoxygenase inhibitory activities of some plant extracts and chemical
compounds. European Journal of Biology, 73 (2), pp. 47-52.
3. Baylac, S., Racine, P. (2003). Inhibition of
5-lipoxygenase by essential oils and other natural fragrant extracts.
International Journal of Aromatherapy, 13 (2-3), pp. 138-142.