Research Information System
Inorganic Chemistry Communications, vol.190, 2026 (SCI-Expanded, Scopus)
The development of highly sensitive and selective fluorescent probes for the detection of biomolecules such as riboflavin (RF) and bilirubin has significant implications for the fields of clinical diagnosis and bioanalysis. In this study, a fluorescence-based sensor for detecting riboflavin (RF) and bilirubin (BR), which play important roles in human health, has been developed. In this context, graphitic carbon nitride (g-C₃N₄) was synthesized using the microwave method, a fast, effective, and innovative technique, with the help of urea and citric acid precursors. The obtained g-C₃N₄ was characterized using Fourier Transform Infrared spectroscopy (FT-IR), UV-VIS spectroscopy, X-ray diffraction (XRD), Scanning electron microscope (SEM), and Fluorescence spectrophotometry (FL) characterization methods. UV-VIS analysis revealed that g-C₃N₄ exhibited absorption peaks at 327 nm and 406 nm, with a band energy calculated as 2.75 eV. RF concentration was studied in the range of 0.1–10 μM; the limit of detection (LOD) was determined to be 2.05 μM, and the limit of quantification (LOQ) was determined to be 6.22 μM. BR concentration was studied in the range of 0.1–10 μM; LOD was determined to be 3.36 μM, and LOQ was determined to be 10.18 μM.