Akademik Veri Yönetim Sistemi
4th International Eurasian Conference on Biological and Chemical Sciences (4.EurasianBioChem-2021), Ankara, Türkiye, 24 - 26 Kasım 2021, cilt.1, sa.1, ss.14
Lycopene, one of the most powerful
carotenoid antioxidants, is widely used with food. Tumor necrosis factor alpha
(TNF-α) is a cell signaling protein involved in systemic inflammation. It is
one of the proinflammatory cytokines that make up the acute phase reaction. It
is an effective and sensitive method in the determination of inflammatory
reactions. We aimed to observe the effect of lycopene on inflammation against
kidney cell damage in which high glucose is applied. NRK-52E cells were
propagated in vitro by regular passages. With the MTT viability test, the
proliferative concentration of lycopene was determined as 1 µM, and the IC50 value
of glucose at 24 hours was determined as 218 mM. Our study consists of 4 groups
at 24 hours: control (C), lycopene (L), high glucose (G), high glucose +
lycopene (GL). Expression levels of TNF-α, one of the inflammation genes, in
RNA isolation products obtained from the groups were investigated by real
time-qPCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the
internal control gene in PCR. According to the findings, it was determined that
high glucose-induced inflammation showed activity in NRK-52E kidney cell line.
It was determined that inflammation increased with cytotoxic effect in G group
and expression decreased in GL group with lycopene administration. As a result,
it was concluded that lycopene provided a benefit due to a decrease in
inflammation gene expression in the 24th hour high glucose environment.
Key
words:
cell line, inflammation, lycopene, high glucose