Effect of lycopene on TNF-α gene expression in high glucose-treated NRK-52E cells


Usta A. , Yüksek V. , Çetin S. , Dede S.

4th International Eurasian Conference on Biological and Chemical Sciences (EurasianBioChem 2021), Ankara, Turkey, 24 - 26 November 2021, vol.1, no.1, pp.14

  • Publication Type: Conference Paper / Summary Text
  • Volume: 1
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.14

Abstract

Lycopene, one of the most powerful carotenoid antioxidants, is widely used with food. Tumor necrosis factor alpha (TNF-α) is a cell signaling protein involved in systemic inflammation. It is one of the proinflammatory cytokines that make up the acute phase reaction. It is an effective and sensitive method in the determination of inflammatory reactions. We aimed to observe the effect of lycopene on inflammation against kidney cell damage in which high glucose is applied. NRK-52E cells were propagated in vitro by regular passages. With the MTT viability test, the proliferative concentration of lycopene was determined as 1 µM, and the IC50 value of glucose at 24 hours was determined as 218 mM. Our study consists of 4 groups at 24 hours: control (C), lycopene (L), high glucose (G), high glucose + lycopene (GL). Expression levels of TNF-α, one of the inflammation genes, in RNA isolation products obtained from the groups were investigated by real time-qPCR. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control gene in PCR. According to the findings, it was determined that high glucose-induced inflammation showed activity in NRK-52E kidney cell line. It was determined that inflammation increased with cytotoxic effect in G group and expression decreased in GL group with lycopene administration. As a result, it was concluded that lycopene provided a benefit due to a decrease in inflammation gene expression in the 24th hour high glucose environment.

Key words: cell line, inflammation, lycopene, high glucose