CYCLOOXYGENASE INHIBITORY ACTIVITY AND COMPOSITION OF ECHINOPHORA TENUIFOLIA L. SUBSP. SIBTHORPIANA (GUSS.) TUTIN L. ESSENTIAL OIL


Yıldız G., Temel H. E., Demirci B.

The Seventh International Mediterranean Symposium on Medicinal and Aromatic Plants, İzmir, Turkey, 18 - 20 November 2021, pp.46

  • Publication Type: Conference Paper / Summary Text
  • City: İzmir
  • Country: Turkey
  • Page Numbers: pp.46
  • Van Yüzüncü Yıl University Affiliated: Yes

Abstract

CYCLOOXYGENASE INHIBITORY ACTIVITY AND COMPOSITION OF ECHINOPHORA TENUIFOLIA L. SUBSP. SIBTHORPIANA (GUSS.) TUTIN L. ESSENTIAL OIL

 

Gulsum Yildiz1, Halide Edip Temel2, Betul Demirci3

 

1 Department of Pharmacognosy, Faculty of Pharmacy, Van Yuzuncu Yil University, 65080, Van, Turkey, gulsumyildiz@yyu.edu.tr

2 Department of Biochemistry, Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Turkey, heincedal@anadolu.edu.tr

3 Department of Pharmacognosy, Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Turkey, bdemirca@anadolu.edu.tr

 

Objective / Purpose: Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin L. (Apiaceae) is known as ‘çörtük, turşu otu, tarhana otu’ and used both food and folk medicine in Turkey [1-3].

The aim of this study was to determine the chemical composition and inhibitory activity of the essential oil on cylooxygenase enzymes of E. tenuifolia subsp. sibthorpiana.

 

Material and Methods: The plant was collected in May 2018, from Tokat province, Turkey. Aerial parts of the plant were subjected to hydrodistillation using Clevenger apparatus. Chemical composition of the oil was analyzed with GC-FID and GC-MS techniques. The effect of inhibition of essential oil on cyclooxygenase enzymes was investigated using the "COX Colorimetric Inhibitor Screening Test Kit" containing both ovine COX-1 and human recombinant COX-2 enzymes.

 

Results: Main components of the oil were found as methyl eugenol (45.5%), p-cymene (11.5%) and δ-3-carene (11.2%). The oil is exhibited inhibitory activity at 100 µg/ml on cyclooxygenase-1 (COX-1); 68,89±2,96 %, while no inhibition activity on cyclooxygenase-2 (COX-2). Rofekoksib and SC-560 were used as standard inhibitors for COX-2 and COX-1, respectively.

 

Conclusion / Discussion: The results showed that the essential oil may play an inhibitory role on COX-1 enzyme and inflammation relation diseases. To the best of our knowledge, this is the first contribution into the COX enzymes inhibition activity of Echinophora tenuifolia L. subsp. sibthorpiana (Guss.) Tutin L. essential oil.

 

Key Words:. Echinophora tenuifolia subsp. sibthorpiana, essential oil, GC, GC/MS, COX

 

Acknowledgement

We are grateful to Prof. Dr. Yavuz Bulent Kose, Department of Pharmaceutical Botany, Faculty of Pharmacy, Anadolu University, Eskişehir, Turkey, for determination of the plant specimen.

 

References

[1] Baytop, T. (1999). Türkiyede bitkiler ile tedavi (geçmişte ve bugün) (No. 40). İstanbul Üniversitesi yay., pp. 166-167.

[2] Melikoğlu, G., Kurtoğlu, S., & Kültür, Ş. (2015). Türkiye’de Astım Tedavisinde Geleneksel Olarak Kullanılan Bitkiler. Marmara Pharmaceutical Journal, 19(1), 1-11.

[3] Bulut, G., Tuzlaci, E., Dogan, A., & Senkardes, I. (2014). An ethnopharmacological review on the Turkish Apiaceae species. Journal of Faculty Pharmacy of Istanbul University, 44(2), 163-179.