The effect of thymoquinone treatment on nuclear factor kappa B (NfKB) and DNA damage on experimental diabetic rats

Usta A., Dede S.

23rd meeting of the Balkan Clinical Laboratory Federation (23.BCLF-2015), Sarajevo, Bosnia And Herzegovina, 7 - 09 October 2015, vol.22, no.1, pp.23

  • Publication Type: Conference Paper / Full Text
  • Volume: 22
  • City: Sarajevo
  • Country: Bosnia And Herzegovina
  • Page Numbers: pp.23
  • Van Yüzüncü Yıl University Affiliated: Yes


Background: In this study, effects of nuclear factor kappa B and formation of DNA damage on detection of the possible occurrence of complications depending on experimental diabetics were investigated. The evaluation of the possible role of thymoquinone (TQ) was aimed in the prevention and treatment of these complications. Materials/subjects and methods: For this purpose, 28 male Wistar-Albino rats weighing between 200-250 g were used. Each containing of seven rats, the rats were divided into four groups of control (C), TQ (T), diabetes (D) and diabetes + TQ (DT). TQ was administered as 30 mg/kg/day by oral gavage to the rats in DT and T groups. In the blood samples collected after 21 days of trial, the values of glucose, HbA1c, ALT, AST, GGT, urea, uric acid, creatinine were measured as well as the quantities of NF-KB and 8-OHdG. Results: It was determined that glucose levels were increased significantly in D group (p <0.05), decreased significantly and approached to control group in DT (p <0.05) and decreased in the group in T group was compared to the control group (p <0.05). HbA1c levels were significantly increased only in the diabetic group (p <0.05), and decreased in DT and approached to the control. It was observed that ALT and AST activities were increased significantly in D group (p <0.05), while significantly decreased in DT group closing to the control. GGT activity was the highest in the D group (p <0.05) but decreased significantly in DT group compared to D group (p <0.05). Urea concentrations were the highest in D and the lowest in T (p <0.05) while decreased significantly in DT group compared to the D group (p <0.05). DNA damage were increased in both of the diabetics, but, was not statistically significant. NFκB levels were the highest in the diabetic group (p <0.05), while there was no important difference in TQ and DT groups compared to the diabetic group. Conclusions: As a result, it was observed that increased glucose and HbA1c levels by STZ-induced diabetes and indicators of liver and kidney damages were decreased significantly and approached to the control group following the administration of TQ. It was determined that 8-OHdG which is an indicator of DNA damage and NFκB levels were increased in the D group.