Akademik Veri Yönetim Sistemi
7. Uluslararası Doğa ve Uygulamalı Bilimlerdeki Gelişmeler Konferansı (ICANAS 24) , Antalya, Türkiye, 17 - 20 Nisan 2024, ss.19-21, (Özet Bildiri)
In this study, the healing effect of the melatonin hormone on the harmful effects of deltamethrin, a pyrethroid pesticide used extensively in the world and in Turkey, on mouse liver cells was investigated. In the study, hepatocytes from Balb/C mice were isolated by a two-stage perfusion method. The proportion of live hepatocytes isolated was over 85%. The isolated cells were cultured with n this study, the healing effect of the melatonin hormone on the harmful effects of deltamethrin, a pyrethroid pesticide used extensively in the world and in Turkey, on mouse liver cells was investigated. In the study, hepatocytes from Balb/C mice were isolated by a two-stage perfusion method. The proportion of live hepatocytes isolated was over 85%. The isolated cells were cultured with different doses of deltamethrin (1 ve 10 µM) and melatonin (100 µM) for 48 hours. At the end of the culture, hepatocytes were extracted at 24th and 48th hours and Malondialdehyde (MDA), Total oxidation (TOS), Total antioxidation (TAS) and DNA damages (8-hydroxy-2'-deoxyguanosine (8-OHdG)) were examined. While an increase in MDA, TOS and DNA damage was observed in the deltamethrin administered groups, a decrease in TAS level was observed. It was determined that the applied deltamethrin had no effect on cell viability throughout the application period. It was observed that melatonin administered together with deltamethrin reduced the toxic effect of deltamethrin. In this study, it can be said that melatonin has a protective effect against deltamethrin-induced damage in mouse hepatocyte cells.different doses of deltamethrin (1 ve 10 µM) and melatonin (100 µM) for 48 n this study, the healing effect of the melatonin hormone on the harmful effects of deltamethrin, a pyrethroid pesticide used extensively in the world and in Turkey, on mouse liver cells was investigated. In the study, hepatocytes from Balb/C mice were isolated by a two-stage perfusion method. The proportion of live hepatocytes isolated was over 85%. The isolated cells were cultured with different doses of deltamethrin (1 ve 10 µM) and melatonin (100 µM) for 48 hours. At the end of the culture, hepatocytes were extracted at 24th and 48th hours and Malondialdehyde (MDA), Total oxidation (TOS), Total antioxidation (TAS) and DNA damages (8-hydroxy-2'-deoxyguanosine (8-OHdG)) were examined. While an increase in MDA, TOS and DNA damage was observed in the deltamethrin administered groups, a decrease in TAS level was observed. It was determined that the applied deltamethrin had no effect on cell viability throughout the application period. It was observed that melatonin administered together with deltamethrin reduced the toxic effect of deltamethrin. In this study, it can be said that melatonin has a protective effect against deltamethrin-induced damage in mouse hepatocyte cells. hours. At the end of the culture, hepatocytes were extracted at 24th and 48th hours and Malondialdehyde (MDA), Total oxidation (TOS), Total antioxidation (TAS) and DNA damages (8-hydroxy-2'- deoxyguanosine (8-OHdG)) were examined. While an increase in MDA, TOS and DNA damage was observed in the deltamethrin administered groups, a decrease in TAS level was observed. It was determined that the applied deltamethrin had no effect on cell viability throughout the application period. It was observed that melatonin administered together with deltamethrin reduced the toxic effect of deltamethrin. In this study, it can be said that melatonin has a protective effect against deltamethrin-induced damage in mouse hepatocyte cells. Acknowledgement: This work was supported by Scientific Research Projects Coordination Unit of Van Yüzüncü Yil University (Project Number: FBA-2022-9901).