In vitro germination and bulblet and shoot propagation for wild edible Eremurus spectabilis M.Bieb.


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Tuncer B.

Notulae Botanicae Horti Agrobotanici Cluj-Napoca, cilt.48, ss.814-825, 2020 (SCI Expanded İndekslerine Giren Dergi)

  • Cilt numarası: 48 Konu: 2
  • Basım Tarihi: 2020
  • Dergi Adı: Notulae Botanicae Horti Agrobotanici Cluj-Napoca
  • Sayfa Sayıları: ss.814-825

Özet

Eremurus spectabilis M.Bieb is consumed as a vegetable because of its nutritious characteristics. The

plants are also used for medicinal purposes, in the cut flower industry as an ornamental geophyte, and in

industry as a natural adhesive. The aim of the present study was to improve the in vitro propagation protocol

for germination and bulblet/shoot formation of E. spectabilis. For this purpose, E. spectabilis seeds were in vitro

germinated in four different nutrient media: Murashige and Skoog (MS), Gamborg (B5), White (WH), and

Shenk and Hildebrandt (SH). To stimulate bulblet and/or shoot regeneration, hypocotyls of 35-40-day-old in

vitro-germinated plantlets were cut into 0.5-1.0 cm pieces, and the resultant explants were cultured in MS

media containing 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5, 1.0, 2.0, and 4.0 mg L-1) + kinetin (0.5 mg L-1),

thidiazuron (TDZ) (0.5, 1.0, 2.0, and 4.0 mg L-1) + 1-naphthylacetic acid (NAA) (0, 0.1, 0.5, and 1.0 mg L-1),

and 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 4.0 mg L-1) + 2,4-dichlorophenoxyacetic acid (2,4-D) (0,

0.1, 0.5, and 1.0 mg L-1). The best outcomes for germination ratio (57.5%) were obtained from the B5 medium.

In the third set of in vitro propagation experiments, 100% bulblet formation was achieved in TDZ (0.5 mg L-

1) and NAA (0.5 and 0.1 mg L-1) combinations of MS media, and this was followed by 0.5 mg L-1 BAPcontaining

medium (81.3%). Shoot formation ratios with the same media combinations varied from 60-70%,

and the number of shoots per explant varied from 1.4-2.4 shoots. Further in vitro propagation research is

planned with larger bulb sizes to develop a protocol for rooting bulblets and/or shoots.

Keywords: Eremurus; geophyte; in vitro regeneration; Liliaceae; plant growth; regulator