In vitro germination and bulblet and shoot propagation for wild edible Eremurus spectabilis M.Bieb.


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Tuncer B.

NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA, vol.48, no.2, pp.814-825, 2020 (Peer-Reviewed Journal) identifier identifier

  • Publication Type: Article / Article
  • Volume: 48 Issue: 2
  • Publication Date: 2020
  • Doi Number: 10.15835/nbha48211883
  • Journal Name: NOTULAE BOTANICAE HORTI AGROBOTANICI CLUJ-NAPOCA
  • Journal Indexes: Science Citation Index Expanded, Scopus, Academic Search Premier, Agricultural & Environmental Science Database, Aqualine, CAB Abstracts, Pollution Abstracts, Veterinary Science Database
  • Page Numbers: pp.814-825
  • Keywords: Eremurus, geophyte, in vitro regeneration, Liliaceae, plant growth, regulator, PLANT-GROWTH REGULATORS, REGENERATION, ANTIOXIDANT, INDUCTION, PROTOCOL

Abstract

Eremurus spectabilis M.Bieb is consumed as a vegetable because of its nutritious characteristics. The plants are also used for medicinal purposes, in the cut flower industry as an ornamental geophyte, and in industry as a natural adhesive. The aim of the present study was to improve the in vitro propagation protocol for germination and bulblet/shoot formation ofE. spectabilis. For this purpose, E. spectabilis seeds were in vitro germinated in four different nutrient media: Murashige and Skoog (MS), Gamborg (B5), White (WH), and Shenk and Hildebrandt (SH). To stimulate bulblet and/or shoot regeneration, hypocotyls of 35-40-day-old in vitro-germinated plantlets were cut into 0.5-1.0 cm pieces, and the resultant explants were cultured in MS media containing 2,4-dichlorophenoxyacetic acid (2,4-D) (0.5, 1.0, 2.0, and 4.0 mg L-1) + kinetin (0.5 mg L-1), thidiazuron (TDZ) (0.5, 1.0, 2.0, and 4.0 mg L-1) + 1-naphthylacetic acid (NAA) (0, 0.1, 0.5, and 1.0 mg L-1), and 6-benzylaminopurine (BAP) (0.5, 1.0, 2.0 and 4.0 mg L-1) + 2,4-dichlorophenoxyacetic acid (2,4-D) (0, 0.1, 0.5, and 1.0 mg L-1). The best outcomes for germination ratio (57.5%) were obtained from the B5 medium. In the third set of in vitro propagation experiments, 100% bulblet formation was achieved in TDZ (0.5 mg L-1) and NAA (0.5 and 0.1 mg L-1) combinations of MS media, and this was followed by 0.5 mg LA BAP-containing medium (81.3%). Shoot formation ratios with the same media combinations varied from 60-70%, and the number of shoots per explant varied from 1.4-2.4 shoots. Further in vitro propagation research is planned with larger bulb sizes to develop a protocol for rooting bulblets and/or shoots.