Cardiomyopathies are the most common type of cardiac diseases in cats. Although some normal echocardiographic values for cats have been published, there are variations based on breeds and gender. The objective of this study is to determine normal reference values for M-mode echocardiographic parameters in nonsedated healthy adult Van cats and to compare those values with data reported for nonsedated healthy cats of other breeds. A total of 40 clinically healthy Van cats of both sexes belonging to the Van Cat Research and Application Center of Yuzuncu Yil University were used. Body weight (BW) and 16 M-mode echocardiographic variables were measured in 40 healthy Van cats. The effect of gender and age on each echocardiographic parameter was analyzed and the relationship between BW and each parameter investigated. There was a significant relationship between gender and left atrial dimension during ventricular systole (LAD) and aortic root dimension at end-diastole (AOD) as well as between BW and interventricular septal thickness at end-diastole (IVSd) and end-systole (IVSs), left ventricular internal dimension at end-diastole (LVIDd), left ventricular posterior wall thickness at end-diastole (LVPWd), LAD, AOD, the left ventricular end diastolic volume (EDV) and the stroke volume (SV). A relationship between age and the SV parameter alone was also established. This present study is the first work on cardiac reference values for Van cats highlighting the differences in some M-mode echocardiographic parameters of healthy adult Van cats and other cat breeds, which should be considered when interpreting echocardiographic findings, in order to draw the correct conclusions regarding cardiac health.