A study on esterase activity of carbonic anhydrase from Van Lake fish (Calcalburnus tarichi)


TESTERECI H., SEKIN S., Ekin S.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.23, ss.145-153, 1999 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 23
  • Basım Tarihi: 1999
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Sayfa Sayıları: ss.145-153

Özet

No study has been reported on carbonic anhydrase enzyme of the fish (Calcalburnus tarichi) living in the carbonated Van Lake. Having partial purification of Carbonic anhydrase from gill and liver of Van Lake fish by ethanol-chloroform and acetone-chloroform, the esterase activity was performed by using p-nitrophenyl acetate as a substrate on spectrophotometer. The effects of the some kinetic parameters (km, Vo, Vmax, Na2CO3 activation, optimum pH etc.) have been examined