The incidence of Barley Yellow Dwarf Viruses (BYDVs) in Wheat Crops in Diyarbakir (Turkey) and Sequence Characterization of BYDV-PAV


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Usta M. , HASSAN N. O. , AL-MAAROOF E. M. , GÜLLER A.

YYÜ TAR BİL DERG (YYU J AGR SCI), cilt.28, ss.140-149, 2018 (Hakemli Üniversite Dergisi)

  • Cilt numarası: 28 Konu: 28
  • Basım Tarihi: 2018
  • Dergi Adı: YYÜ TAR BİL DERG (YYU J AGR SCI)
  • Sayfa Sayısı: ss.140-149

Özet

To ascertain the presence of Barley/Cereal yellow dwarf viruses (BYDV-PAV, MAV, SGV, RMV, and CYDV-RPV), a total of 365 wheat leaf samples were randomly collected from wheat cultivation fields of Diyarbakir province in 2016 and were screened by using multiplex RT-PCR and RT-PCR methods. As a result of PCR tests the wheat samples were found to be infected by BYDV-PAV, BYDV-SGV and CYDV-RPV with the percentage of 3.5%, 2.4% and 1.3%, respectively. Detected mix infections of BYDV-PAV+BYDV-SGV, BYDV-PAV+CYDV-RPV,  BYDV-SGV+CYDV-RPV,  and  BYDV-PAV+BYDV-SGV+CYDV-RPV  were 2.2%,  0.8%,  0.8%,  and  0.8%,  respectively. A  BYDV-PAV  Diyarbakir  isolate  was  chosen  randomly,  and  its complete coat protein gene was characterized. The coat protein gene of BYDV-PAV virus (Genbank accession no. KX774424) was molecularly cloned and sequenced with the universal primers. The CP nucleotide sequence of BYDV-PAV Diyarbakir isolate  was showed 81.4 - 98.3% identities  with the other 21 world isolates. The present  study  is  the  first  report  for  documentation  of  BYDV-PAV,  BYDV-SGV  and  CYDV-RPV  viruses  in
wheat fields of Diyarbakir province (Turkey).