Investigation of age-related histological hepatic alterations after administration of intermittent fasting

Ceylani T., Teker H. T., Eranıl I., Yılmaz Ertürk F., Keskin S.

1st International Congress on Solutions in Science (INSCORE), İzmir, Turkey, 11 - 13 September 2023, pp.1

  • Publication Type: Conference Paper / Full Text
  • City: İzmir
  • Country: Turkey
  • Page Numbers: pp.1
  • Van Yüzüncü Yıl University Affiliated: Yes


Intermittent fasting (IF) is a preferred dietary approach in preventing or delaying age-related diseases due to its effects on lifespan and regulation of cellular damage. IF is an important nutritional strategy for improving multiple features of hepatometabolic and age-related pathologies, due to weight and insulin resistance reduction. This study aimed to evaluate possible histoprotective effects of IF on age-related rat liver. In this study, twelve rats were divided into two groups: Nonfasting Group (12 months) without IF and the Fasting Group (12 months, for 35 days). The Fasting group's access interval to food was between 09.00 am and 15.00 pm. In addition, their access to food was restricted for 18 hours. At the end of the experiment, the liver sections were stained with H&E and Masson trichrome, and then evaluated for histopathological examination by light microscopy. In the H&E analysis, there was a significant increase in inflammation in the lobular and portal areas, spotty necrosis, and balloon degeneration in the Nonfasting group compared to the Fasting group. In addition, Masson Trichrome staining results showed a significant decrease in the density of collagen deposition in the connective tissue of the liver in the Fasting group compared to the Nonfasting group. Intermittent fasting may help improve the morphological changes that happen as the liver ages by reducing cell damage in the aged liver histological structure.7

Keywords: Aging, inflammation, intermitted fasting, liver, histopathology