Detection and RFLP analysis of canine parvovirus (CPV) DNA by polymerase chain reaction (PCR) in a dog


Ozkul A., Keles I., Karaoglu T., Cabalar M., Burgu I.

TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES, cilt.26, ss.1201-1203, 2002 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 26 Konu: 5
  • Basım Tarihi: 2002
  • Dergi Adı: TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
  • Sayfa Sayıları: ss.1201-1203

Özet

In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.