New Diagnostic Biomarker-Soluble Erythropoietin-producing hepatocellular receptor A2 (EphA2) for colon cancer

İliklerden Ü. H., Kalayci T.

INDIAN JOURNAL OF SURGERY, vol.85, no.2, pp.301-306, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 85 Issue: 2
  • Publication Date: 2023
  • Doi Number: 10.1007/s12262-022-03360-0
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, CAB Abstracts, CINAHL, Veterinary Science Database
  • Page Numbers: pp.301-306
  • Keywords: Erythropoietin-producing hepatocellular receptor A(2), EphA(2), Colon cancer, Diagnosis, EPHRINS
  • Van Yüzüncü Yıl University Affiliated: Yes


To evaluate the association between soluble erythropoietin-producing hepatocellular receptor A(2) (EphA(2)) levels and the patients' tumor stage, tumor size, and lymph node metastasis. This study was planned at a tertiary health institute from May 2020 to October 2020 as prospective observational study after ethical committee approval. Samples were taken from 90 colon cancer patients and 40 healthy volunteers matched for age and sex. All samples were centrifuged to eliminate components and stored at -80 degrees C until testing. Serum levels of the EphA(2) were measured with sandwich ELISA. The mean age of the control group was 63.48 years (55-0 years; 19 men and 21 women), while the mean age of patient's group was 64.09 (range from 57 to 70 years; 46 men and 44 women). There was no difference between patient and control group according to age and gender (p>0.05). The mean value of serum EphA(2) was 194.75 ng/mL in the control group and 2622.39 ng/mL in the colon cancer patients (p<0.001). EPHA(2) level was significantly higher in the patients with tumor diameter over 30 mm (p<0.001). In addition, EPHA(2) levels were higher in patients with advanced TNM stage, deeply invasive tumors, multiple lymph node metastases, and distant metastasis (p<0.001). From the result of this study, EPHA(2) levels can be a diagnostic marker and can be used to differentiate cancer stages (to distinguish stages I-III from stage IV). It is also the first study investigating the relationship between EPHA(2) levels and colon cancer.