KAFKAS UNIVERSITESI VETERINER FAKULTESI DERGISI, cilt.26, sa.6, ss.757-763, 2020 (SCI Expanded İndekslerine Giren Dergi)
Cyclophosphamide (CD) has a range of adverse effects on ovarian tissue in humans. It is widely an anticancer drug and used in autoimmune
disorders. Also, CD produces reactive oxygen species. In the current study, we evaluated possible protective roles of Rhabdosciadium
anatolyi (RA) and chlorogenic acid (CA) on histopathological alterations and immunohistochemical assessment of 8-OHdG in the ovarian
tissues of female rats given cyclophosphamide. Female Wistar albino rats were divided into six experimental groups, each consisted of eight
rats: control group, CD (200 mg/kg) with i.p. for the first day as single dose, CA (100 mg/kg), RA (300 mg/kg) for each day, RA (300 mg/kg) +
CD (200 mg/kg) and CA(100 mg/kg) + CD (200 mg/kg) groups. The rats were administered treatments lasted 7 days for all groups. CA and
RA treatment is associated with positive the ovary action CD-induced ovotoxicity in rats. CA and RA could ameliorate the histopathological
and immunohistochemical finding restoring which may show moderate levels of primordial follicle, primary, secondary, tertiary and graafian
follicles were observed degeneration in germinative cells. Therefore, our results suggest that chlorogenic acid and Rhabdosciadium anatolyi
might be a protective effect for CD-induced ovotoxicity.