Molecular analysis of 'Candidatus Phytoplasma trifolii' and 'Candidatus Phytoplasma solani' associated with phytoplasma diseases of tomato (PDT) in Turkey


Creative Commons License

Usta M. , Güller A., Sipahioglu H. M.

International Journal of Agriculture and Biology, vol.20, no.9, pp.1991-1996, 2018 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 9
  • Publication Date: 2018
  • Doi Number: 10.17957/ijab/15.0721
  • Title of Journal : International Journal of Agriculture and Biology
  • Page Numbers: pp.1991-1996
  • Keywords: 'Candidatus Phytoplasma trifolii', 'Candidatus Phytoplasma solani', Molecular analysis, Solanum lycopersicum, BIG BUD DISEASE, CLASSIFICATION, IDENTIFICATION, AMPLIFICATION, PLANTS, CROPS, RFLP

Abstract

Tomato plants displaying severe fruit deformation, flower sterility, aerial rooting, purplish leaves and leaf rolling were
observed in tomato fields at Van province (Turkey). Samples were collected, and total DNA was extracted from symptomatic
and asymptomatic plants. Nested polymerase chain reaction (nested-PCR) assays were performed to amplify 16S rDNA
sequences for molecular detection using universal primer pairs. Out of 100 tested tomato samples, 11% of tomato samples
yielded a DNA fragment of 1.25 kb. Amplified PCR products were then cloned into pGEM T-Easy vector and sequenced
using new generation DNA sequencing (NGS) system. The virtual restriction fragment length polymorphism (RFLP) analysis
of 16S rDNA sequences and molecular detections were allowed to characterize possible phytoplasmas associated with
diseased plants. Our results revealed the presence of two
Phytoplasma species belonging to two different ribosomal groups;
Candidatus Phytoplasma trifolii‟ (16Sr VI-A group) (Acces no. MF564268, MG732925) and „Candidatus Phytoplasma
solani
‟ (16SrXII-A group) (Acces no. KY579358, MF576263). Despite a high variation in their similarity coefficient of „Ca.
P. solani‟ VTS2 (0.91) and „Ca. P. trifolii‟ VTT1 (0.88) isolates, the infected tomato plants generally displayed similar disease
symptoms during field observations
. Due to its commercial interest, co-existing of these phytoplasmas in tomato fields is of
great phytosanitary significance not only for tomato plants but also for other crops such as vegetables, ornamentals and field
crops. With this study, „
Ca. P. trifolii‟ associated with phytoplasma diseases of tomato (PDT) has been reported for the first
time in tomato in Turkey.